Goré J, Hoinard C, Maingault P
Biochim Biophys Acta. 1986 Apr 14;856(2):357-61. doi: 10.1016/0005-2736(86)90046-5.
Isolated intestinal mucosa cells of rats were used to investigate the intestinal transport of biotin. This method utilizing a double-label isotope technique showed that uptake could not be saturated, even in a wide range of biotin concentrations (0.01-2 microM). A metabolic inhibitor (antimycin A) did not prevent cell uptake of biotin. The transport mechanism was independent of temperature (Q10 = 1.04). When excess biotin was added to the incubation medium, there was no efflux of the vitamin from intestinal cells. The results also showed that the cells did not concentrate the vitamin, regardless of its concentration in the incubation medium. The mechanism of biotin uptake by rat cells at physiological concentrations is thus a passive diffusion phenomenon.
采用大鼠离体肠黏膜细胞研究生物素的肠道转运。该方法运用双标记同位素技术，结果显示即便在生物素浓度范围很宽（0.01 - 2微摩尔）的情况下，摄取也不会饱和。代谢抑制剂（抗霉素A）并不能阻止细胞摄取生物素。转运机制与温度无关（温度系数Q10 = 1.04）。当向孵育培养基中添加过量生物素时，维生素并未从肠细胞中流出。结果还表明，无论孵育培养基中维生素的浓度如何，细胞都不会浓缩该维生素。因此，生理浓度下大鼠细胞摄取生物素的机制是一种被动扩散现象。
