Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1492-1498. doi: 10.1099/ijs.0.045054-0. Epub 2012 Aug 10.
Two strictly anaerobic bacterial strains, designated IP3-3(T) and SBP-1, were isolated from groundwater contaminated by chlorinated alkanes and alkenes at a Superfund Site located near Baton Rouge, Louisiana (USA). Both strains reductively dehalogenate a variety of polychlorinated aliphatic alkanes, including 1,2-dichloroethane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2,3-trichloropropane, when provided with hydrogen as the electron donor. To clarify their taxonomic position, strains IP3-3(T) and SBP-1 were characterized using a polyphasic approach. Both IP3-3(T) and SBP-1 are mesophilic, non-spore-forming, non-motile and Gram-stain-negative. Cells of both strains are irregular cocci with diameters of 0.4-1.1 µm. Both are resistant to ampicillin and vancomycin. The genomic DNA G+C contents of strains IP3-3(T) and SBP-1 are 55.5±0.4 and 56.2±0.2 mol% (HPLC), respectively. Major cellular fatty acids include C18 : 1ω9c, C16 : 0, C14 : 0 and C16 : 1ω9c. 16S rRNA gene sequence based phylogenetic analyses indicated that the strains cluster within the phylum Chloroflexi most closely related to but distinct from the species Dehalogenimonas lykanthroporepellens (96.2 % pairwise similarity) and Dehalococcoides mccartyi (90.6 % pairwise similarity). Physiological and chemotaxonomic traits as well as phylogenetic analysis support the conclusion that these strains represent a novel species within the genus Dehalogenimonas for which the name Dehalogenimonas alkenigignens sp. nov. is proposed. The type strain is IP3-3(T) ( = JCM 17062(T) = NRRL B-59545(T)).
两株严格厌氧细菌菌株，分别命名为 IP3-3(T) 和 SBP-1，从路易斯安那州巴吞鲁日附近一个超级基金场地受氯代烷烃和烯烃污染的地下水分离得到。这两株菌在提供氢气作为电子供体时，可以还原脱卤化各种多氯代脂肪族烷烃，包括 1,2-二氯乙烷、1,2-二氯丙烷、1,1,2,2-四氯乙烷、1,1,2-三氯乙烷和 1,2,3-三氯丙烷。为了阐明它们的分类地位，使用多相方法对菌株 IP3-3(T) 和 SBP-1 进行了表征。IP3-3(T) 和 SBP-1 均为中温、非孢子形成、非运动和革兰氏阴性。两株菌的细胞均为直径 0.4-1.1 µm 的不规则球菌。两株菌均对氨苄西林和万古霉素有抗性。菌株 IP3-3(T) 和 SBP-1 的基因组 DNA G+C 含量分别为 55.5±0.4 和 56.2±0.2 mol%（HPLC）。主要细胞脂肪酸包括 C18 : 1ω9c、C16 :0、C14 :0 和 C16 :1ω9c。基于 16S rRNA 基因序列的系统发育分析表明，这些菌株在 Chloroflexi 门内聚类，与 Dehalogenimonas lykanthroporepellens（96.2% 序列相似性）和 Dehalococcoides mccartyi（90.6% 序列相似性）最为密切相关，但又有所不同。生理和化学分类特征以及系统发育分析支持这些菌株代表 Dehalogenimonas 属中的一个新种的结论，建议将其命名为 Dehalogenimonas alkenigignens sp. nov.。模式菌株为 IP3-3(T)（ = JCM 17062(T) = NRRL B-59545(T)）。
