Efimochkina N R, Batishcheva S Iu, Bykova I B, Sheveleva S A
Vopr Pitan. 2012;81(3):24-9.
The data on nomenclature, classification and taxonomy of aerobic spore-forming Bacillus cereus are summarized. The main features of the two types of diseases, caused B. cereus, and statistical information on outbreaks of food-borne disease caused by B. cereus are presented. The detailed description of emetic toxin cereulide properties are given. The analysis of existing methods for detection of the presence of B. cereus and their toxins in foods are conducted. The data on the use of different cell models for studying the cytotoxic effects and the enterotoxigenic properties of B. cereus are described. Results of own researches allowed to conclude that certain types of products, primarily made from milk and vegetable raw materials, can be a source of transmission to humans of toxins produced by B. cereus. It is shown that in the absence of competing vegetative microflora increases the risk of accumulation of toxins produced by the most stable populations, including toxigenic spore B. cereus. Tested and proposed for the practical implementation of the dry culture media on the basis of the balanced growth and selective components, dyes and buffer mixtures. The developed environment were used for the isolation and identification B. cereus during microbial control these groups of foods.
总结了蜡样芽孢杆菌需氧芽孢杆菌命名、分类和分类学的数据。介绍了蜡样芽孢杆菌引起的两种疾病的主要特征以及由蜡样芽孢杆菌引起的食源性疾病暴发的统计信息。给出了呕吐毒素蜡样芽胞杆菌毒素特性的详细描述。对食品中蜡样芽孢杆菌及其毒素检测的现有方法进行了分析。描述了使用不同细胞模型研究蜡样芽孢杆菌细胞毒性作用和产肠毒素特性的数据。自身研究结果表明,某些主要由牛奶和蔬菜原料制成的产品可能是蜡样芽孢杆菌产生的毒素传播给人类的来源。结果表明,在没有竞争性营养微生物群落的情况下,包括产毒芽孢蜡样芽孢杆菌在内的最稳定菌群产生的毒素积累风险增加。基于平衡生长和选择性成分、染料和缓冲混合物,测试并提出了用于实际应用的干燥培养基。在对这些食品组进行微生物控制期间,所开发的培养基用于蜡样芽孢杆菌的分离和鉴定。