人乳头瘤病毒 18 蛋白 E7 的保守区 2 中的 PxDLLCxE 序列对于 E7 与着丝粒蛋白 C 的结合是必需的。

The PxDLLCxE sequence in conserved region 2 of human papilloma virus 18 protein E7 is required for E7 binding to centromere protein C.

机构信息

Department of Oncology, Graduate School of Health Sciences, Faculty of Life Sciences, Kumamoto University, Chuouku, Kumamoto, Japan.

出版信息

Oncology. 2012;83(4):210-7. doi: 10.1159/000341360. Epub 2012 Aug 11.

DOI:10.1159/000341360

PMID:22890155

Abstract

High-risk human papillomaviruses (HPVs) are etiologically linked to human cervical and oral cancers. HPV infection leads to aneuploidy, a numerical chromosomal aberration caused by dysregulation of chromosomal segregation. We found that high-risk HPV18 and HPV58 E7 proteins bind to centromere protein C (CENP-C), a component of the kinetochore that is essential for proper chromosomal segregation. Low-risk HPV4, HPV6, and HPV11 E7s do not bind to CENP-C. The PxDLLCxE sequence in conserved region 2 (CR2) of HPV18 E7 is required for E7 binding to CENP-C. Our results indicate that differences in the ability of high- and low-risk HPV E7s to bind to CENP-C reflect the different oncogenic potentials of high- and low-risk type HPVs.

摘要

高危型人乳头瘤病毒（HPV）与人类宫颈癌和口腔癌在病因上有关联。HPV 感染会导致非整倍体，即染色体分离失调导致的染色体数目异常。我们发现，高危型 HPV18 和 HPV58 的 E7 蛋白与着丝粒蛋白 C（CENP-C）结合，CENP-C 是动粒的一个组成部分，对于正确的染色体分离至关重要。低危型 HPV4、HPV6 和 HPV11 的 E7 则不与 CENP-C 结合。HPV18 E7 的保守区 2（CR2）中的 PxDLLCxE 序列是 E7 与 CENP-C 结合所必需的。我们的结果表明，高危型和低危型 HPV E7 结合 CENP-C 的能力差异反映了高危型和低危型 HPV 的不同致癌潜力。