Venkatesan S, Rideout J M, Simpson K J
Division of Clinical Sciences and Clinical Cell Biology, Clinical Research Centre, Harrow, Middlesex, UK.
Biomed Chromatogr. 1990 Nov;4(6):234-8. doi: 10.1002/bmc.1130040605.
In experimentally produced alcoholic fatty liver microsomal fatty acid composition was measured using gas chromatography. The results showed an increase in linoleic acid (18:2, n-6) and hexadecaenoic acid (22:6, n-3) and a decrease in arachidonic acid (20:4, n-6) in alcohol-fed rats. Using high performance liquid chromatographic separation of radiolabelled substrate and products, delta 9, delta 6 and delta 5 desaturase enzymes were assayed. The activity of delta 9 and delta 5 desaturase was decreased in alcohol-fed rats and delta 6 desaturase activity was similar in control and alcohol-fed groups. These results indicated there was no causal relationship between desaturase activity and membrane fatty acid changes. Increased amounts of eicosatrienoic acid (20:3, n-9) in rats fed less than 5% fat were observed in both control and alcohol-fed rats. The results indicated that essential fatty acid deficiency was not due to alcohol consumption.
在实验性诱导的酒精性脂肪肝中，使用气相色谱法测定微粒体脂肪酸组成。结果显示，喂食酒精的大鼠体内亚油酸（18:2，n-6）和十六碳六烯酸（22:6，n-3）增加，花生四烯酸（20:4，n-6）减少。使用高效液相色谱法分离放射性标记的底物和产物，对Δ9、Δ6和Δ5去饱和酶进行测定。喂食酒精的大鼠中Δ9和Δ5去饱和酶的活性降低，而对照组和喂食酒精组的Δ6去饱和酶活性相似。这些结果表明，去饱和酶活性与膜脂肪酸变化之间没有因果关系。在对照组和喂食酒精的大鼠中均观察到，喂食脂肪含量低于5%的大鼠体内二十碳三烯酸（20:3，n-9）含量增加。结果表明，必需脂肪酸缺乏并非由酒精摄入所致。
