Bordoni A, Biagi P L, Turchetto E, Hrelia S
Centro Ricerche sulla Nutrizione-Dip.Biochimica, Bologna, Italy.
Biochem Int. 1988 Dec;17(6):1001-9.
The activity of delta-6-desaturase (D6D) in liver microsomes and fatty acid composition of microsomal lipids of rats of different ages were studied. The D6D activity was similar in suckling rats and in weaning rats. However, the enzyme showed a significantly decreased activity in oldest animals, and a linear correlation was found between the D6D activity and the animal age. The fatty acid composition data on total lipids of liver microsomes were consistent with the age-dependent changes in fatty acid desaturase activity. The major changes occurred in the linoleate and arachidonate fractions; the 20:4/18:2 ratio in liver microsomes decreased together with D6D activity during aging. The loss of D6D activity may be a key factor in aging through altering lipid membrane composition.
研究了不同年龄大鼠肝脏微粒体中δ-6-去饱和酶（D6D）的活性以及微粒体脂质的脂肪酸组成。哺乳期大鼠和断奶大鼠的D6D活性相似。然而，该酶在最年长的动物中活性显著降低，并且发现D6D活性与动物年龄之间存在线性相关性。肝脏微粒体总脂质的脂肪酸组成数据与脂肪酸去饱和酶活性的年龄依赖性变化一致。主要变化发生在亚油酸和花生四烯酸部分；随着衰老，肝脏微粒体中的20:4/18:2比值与D6D活性一起降低。D6D活性的丧失可能是通过改变脂质膜组成而导致衰老的关键因素。
