拓扑异构酶II的DNA门打开和关闭的速率。
The rate of opening and closing of the DNA gate for topoisomerase II.
作者信息
Ding Hui, Lin Hao, Feng Juan
机构信息
Key Laboratory for NeuroInformation of Ministry of Education, Center of Bioinformatics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
出版信息
Theory Biosci. 2013 Mar;132(1):61-4. doi: 10.1007/s12064-012-0163-2. Epub 2012 Aug 14.
Type II DNA topoisomerases can catalyze the transport of one DNA segment through a transient break in another DNA segment by a complex mechanism of ATP hydrolysis. According to the hydrolysis process of two ATPs, a multi-state model is proposed to investigate the work cycle of DNA topoisomerase II. The rate of the opening and closing of the DNA topoisomerase gate is evaluated by determining the release rate of inorganic phosphates. The calculated results show that, under the condition of the high concentration of ATP, the work cycle of DNA topoisomerase II is about 0.84 s which is in agreement with the experimental data.
II型DNA拓扑异构酶可以通过一种复杂的ATP水解机制催化一个DNA片段通过另一个DNA片段中的瞬时断裂进行转运。根据两个ATP的水解过程，提出了一个多状态模型来研究DNA拓扑异构酶II的工作循环。通过测定无机磷酸盐的释放速率来评估DNA拓扑异构酶门的打开和关闭速率。计算结果表明，在高浓度ATP条件下，DNA拓扑异构酶II的工作循环约为0.84秒，这与实验数据一致。
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