• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

开放染色质结构调节 Epstein-Barr 病毒中前复制复合物形成和复制起始的效率。

Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus.

机构信息

DNA Replication and Epigenetics group, Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, 81377 München, Germany.

出版信息

J Cell Biol. 2012 Aug 20;198(4):509-28. doi: 10.1083/jcb.201109105. Epub 2012 Aug 13.

DOI:10.1083/jcb.201109105
PMID:22891264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3514025/
Abstract

Whether or not metazoan replication initiates at random or specific but flexible sites is an unsolved question. The lack of sequence specificity in origin recognition complex (ORC) DNA binding complicates genome-scale chromatin immunoprecipitation (ChIP)-based studies. Epstein-Barr virus (EBV) persists as chromatinized minichromosomes that are replicated by the host replication machinery. We used EBV to investigate the link between zones of pre-replication complex (pre-RC) assembly, replication initiation, and micrococcal nuclease (MNase) sensitivity at different cell cycle stages in a genome-wide fashion. The dyad symmetry element (DS) of EBV's latent origin, a well-established and very efficient pre-RC assembly region, served as an internal control. We identified 64 pre-RC zones that correlate spatially with 57 short nascent strand (SNS) zones. MNase experiments revealed that pre-RC and SNS zones were linked to regions of increased MNase sensitivity, which is a marker of origin strength. Interestingly, although spatially correlated, pre-RC and SNS zones were characterized by different features. We propose that pre-RCs are formed at flexible but distinct sites, from which only a few are activated per single genome and cell cycle.

摘要

真核生物的复制起始是随机的还是特定但灵活的位点，这是一个尚未解决的问题。原点识别复合物 (ORC) DNA 结合缺乏序列特异性，这使得基于大规模染色质免疫沉淀 (ChIP) 的研究变得复杂。 Epstein-Barr 病毒 (EBV) 以染色质化的微染色体形式存在，这些微染色体由宿主复制机制复制。我们使用 EBV 来研究在整个基因组范围内，不同细胞周期阶段的前复制复合物 (pre-RC) 组装、复制起始和微球菌核酸酶 (MNase) 敏感性之间的联系。 EBV 潜伏原点的二分对称元件 (DS) 是一个成熟且非常有效的 pre-RC 组装区域，可用作内部对照。我们鉴定了 64 个与 57 个短新生链 (SNS) 区空间相关的 pre-RC 区。MNase 实验表明，pre-RC 和 SNS 区与 MNase 敏感性增加的区域相关，这是原点强度的标志。有趣的是，尽管空间上相关，但 pre-RC 和 SNS 区具有不同的特征。我们提出，pre-RC 是在灵活但独特的位点形成的，每个基因组和细胞周期只有少数几个被激活。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/2b319cf3f584/JCB_201109105_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/dd9dec138d27/JCB_201109105_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/6f488ddd8a29/JCB_201109105_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/03b17f6d6061/JCB_201109105_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/40a7cb7598f5/JCB_201109105_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/8844a53c2565/JCB_201109105_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/9493c8d96f17/JCB_201109105_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/20eb28ffa8ed/JCB_201109105_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/2b319cf3f584/JCB_201109105_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/dd9dec138d27/JCB_201109105_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/6f488ddd8a29/JCB_201109105_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/03b17f6d6061/JCB_201109105_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/40a7cb7598f5/JCB_201109105_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/8844a53c2565/JCB_201109105_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/9493c8d96f17/JCB_201109105_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/20eb28ffa8ed/JCB_201109105_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ca6/3514025/2b319cf3f584/JCB_201109105_Fig8.jpg

相似文献

1
Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus.开放染色质结构调节 Epstein-Barr 病毒中前复制复合物形成和复制起始的效率。
J Cell Biol. 2012 Aug 20;198(4):509-28. doi: 10.1083/jcb.201109105. Epub 2012 Aug 13.
2
The dyad symmetry element of Epstein-Barr virus is a dominant but dispensable replication origin.EB 病毒的二联体对称元素是一个显性但可有可无的复制原点。
PLoS One. 2011;6(5):e18609. doi: 10.1371/journal.pone.0018609. Epub 2011 May 16.
3
Structural Basis for Cooperative Binding of EBNA1 to the Epstein-Barr Virus Dyad Symmetry Minimal Origin of Replication.EBNA1 与 Epstein-Barr 病毒二联体对称最小复制原点协同结合的结构基础。
J Virol. 2019 Sep 30;93(20). doi: 10.1128/JVI.00487-19. Print 2019 Oct 15.
4
Complex protein-DNA dynamics at the latent origin of DNA replication of Epstein-Barr virus.爱泼斯坦-巴尔病毒DNA复制潜在起始位点处复杂的蛋白质-DNA动态变化
J Cell Sci. 2003 Oct 1;116(Pt 19):3971-84. doi: 10.1242/jcs.00708.
5
Structural and Functional Basis for an EBNA1 Hexameric Ring in Epstein-Barr Virus Episome Maintenance.EB病毒游离体维持中EBNA1六聚体环的结构与功能基础
J Virol. 2017 Sep 12;91(19). doi: 10.1128/JVI.01046-17. Print 2017 Oct 1.
6
The BHLF1 Locus of Epstein-Barr Virus Contributes to Viral Latency and B-Cell Immortalization.EB 病毒 BHLF1 基因座促进病毒潜伏期和 B 细胞永生化。
J Virol. 2020 Aug 17;94(17). doi: 10.1128/JVI.01215-20.
7
Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein-Barr virus.人类起源识别复合体与爱泼斯坦-巴尔病毒DNA复制潜在起源区域结合。
EMBO J. 2001 Aug 15;20(16):4588-602. doi: 10.1093/emboj/20.16.4588.
8
Initiation of DNA replication within oriP is dispensable for stable replication of the latent Epstein-Barr virus chromosome after infection of established cell lines.在感染已建立的细胞系后，oriP内DNA复制的起始对于潜伏性爱泼斯坦-巴尔病毒染色体的稳定复制并非必需。
J Virol. 2000 Sep;74(18):8563-74. doi: 10.1128/jvi.74.18.8563-8574.2000.
9
A Mechanism-Based Targeted Screen To Identify Epstein-Barr Virus-Directed Antiviral Agents.一种基于机制的靶向筛选方法，用于鉴定 Epstein-Barr 病毒导向的抗病毒药物。
J Virol. 2020 Oct 14;94(21). doi: 10.1128/JVI.01179-20.
10
Histone Loaders CAF1 and HIRA Restrict Epstein-Barr Virus B-Cell Lytic Reactivation.组蛋白加载器 CAF1 和 HIRA 限制 EBV B 细胞裂解激活。
mBio. 2020 Oct 27;11(5):e01063-20. doi: 10.1128/mBio.01063-20.

引用本文的文献

1
Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones.人类 ORC/MCM 密度在活跃基因中较低，与复制时间相关，但不限制起始区域。
Elife. 2021 Mar 8;10:e62161. doi: 10.7554/eLife.62161.
2
Bacterial artificial chromosomes establish replication timing and sub-nuclear compartment de novo as extra-chromosomal vectors.细菌人工染色体作为额外染色体载体，可从头建立复制时程和亚核区室。
Nucleic Acids Res. 2018 Feb 28;46(4):1810-1820. doi: 10.1093/nar/gkx1265.
3
The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2.

本文引用的文献

1
Chromatin architectures at fission yeast transcriptional promoters and replication origins.裂殖酵母转录启动子和复制原点处的染色质结构。
Nucleic Acids Res. 2012 Aug;40(15):7176-89. doi: 10.1093/nar/gks351. Epub 2012 May 9.
2
Genome-wide identification and characterization of replication origins by deep sequencing.通过深度测序进行全基因组复制起点的鉴定和特征分析。
Genome Biol. 2012 Apr 24;13(4):R27. doi: 10.1186/gb-2012-13-4-r27.
3
Genome-scale analysis of metazoan replication origins reveals their organization in specific but flexible sites defined by conserved features.
乳头瘤病毒E2蛋白与复制起始因子ORC2结合的复制后果
PLoS Pathog. 2016 Oct 4;12(10):e1005934. doi: 10.1371/journal.ppat.1005934. eCollection 2016 Oct.
4
Orc5 induces large-scale chromatin decondensation in a GCN5-dependent manner.Orc5以GCN5依赖的方式诱导大规模染色质解聚。
J Cell Sci. 2016 Jan 15;129(2):417-29. doi: 10.1242/jcs.178889. Epub 2015 Dec 7.
5
Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture.与Cdt1结合的蛋白GRWD1是一种新型组蛋白结合蛋白，它通过影响染色质结构促进微小染色体维持蛋白（MCM）的装载。
Nucleic Acids Res. 2015 Jul 13;43(12):5898-911. doi: 10.1093/nar/gkv509. Epub 2015 May 18.
6
Cis-acting DNA sequence at a replication origin promotes repeat expansion to fragile X full mutation.复制起点处的顺式作用DNA序列促进重复序列扩展至脆性X全突变。
J Cell Biol. 2014 Sep 1;206(5):599-607. doi: 10.1083/jcb.201404157.
7
Fidelity of end joining in mammalian episomes and the impact of Metnase on joint processing.哺乳动物附加体中末端连接的保真度以及金属酶对连接过程的影响。
BMC Mol Biol. 2014 Mar 22;15:6. doi: 10.1186/1471-2199-15-6.
8
High-resolution analysis of DNA synthesis start sites and nucleosome architecture at efficient mammalian replication origins.高效哺乳动物复制起始点处 DNA 合成起始位点和核小体结构的高分辨率分析。
EMBO J. 2013 Oct 2;32(19):2631-44. doi: 10.1038/emboj.2013.195. Epub 2013 Aug 30.
9
The origin recognition complex in human diseases.人类疾病中的原点识别复合物。
Biosci Rep. 2013 Jun 11;33(3):e00044. doi: 10.1042/BSR20130036.
真核生物复制起点的全基因组分析揭示了它们在特定但灵活的位点的组织，这些位点由保守特征定义。
Genome Res. 2011 Sep;21(9):1438-49. doi: 10.1101/gr.121830.111. Epub 2011 Jul 12.
4
Occupancy of chromatin organizers in the Epstein-Barr virus genome.染色质组织者在 Epstein-Barr 病毒基因组中的占据。
Virology. 2011 Jun 20;415(1):1-5. doi: 10.1016/j.virol.2011.04.004. Epub 2011 May 8.
5
Cdc45 limits replicon usage from a low density of preRCs in mammalian cells.Cdc45 限制了哺乳动物细胞中低浓度前 RC 引发的复制起点使用。
PLoS One. 2011 Mar 1;6(3):e17533. doi: 10.1371/journal.pone.0017533.
6
Chromatin signatures of the Drosophila replication program.果蝇复制程序的染色质特征。
Genome Res. 2011 Feb;21(2):164-74. doi: 10.1101/gr.116038.110. Epub 2010 Dec 22.
7
Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.复制前复合物蛋白在中华仓鼠二氢叶酸还原酶起始区的核小体低占有率区域组装。
Nucleic Acids Res. 2011 Apr;39(8):3141-55. doi: 10.1093/nar/gkq1276. Epub 2010 Dec 9.
8
Eukaryotic DNA replication origins: many choices for appropriate answers.真核生物 DNA 复制起始点：多种选择，适者生存。
Nat Rev Mol Cell Biol. 2010 Oct;11(10):728-38. doi: 10.1038/nrm2976.
9
Diversity of eukaryotic DNA replication origins revealed by genome-wide analysis of chromatin structure.通过对染色质结构的全基因组分析揭示真核 DNA 复制起点的多样性。
PLoS Genet. 2010 Sep 2;6(9):e1001092. doi: 10.1371/journal.pgen.1001092.
10
Evaluating genome-scale approaches to eukaryotic DNA replication.评估真核生物 DNA 复制的基因组规模方法。
Nat Rev Genet. 2010 Oct;11(10):673-84. doi: 10.1038/nrg2830. Epub 2010 Sep 1.