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在乳腺上皮细胞中同时进行单通道记录的细胞内钙变化实时成像。

Real-time imaging of intracellular calcium change with simultaneous single channel recording in mammary epithelial cells.

作者信息

Furuya K, Enomoto K

机构信息

Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan.

出版信息

Brain Res Bull. 1990 Nov;25(5):779-81. doi: 10.1016/0361-9230(90)90058-8.

DOI:10.1016/0361-9230(90)90058-8
PMID:2289167
Abstract

A method of continuous image subtraction of fura-2 fluorescence made it possible to observe real-time (video rate) changes in intracellular calcium (Cai). This simple method is very useful for simultaneous measurements in electrophysiology with a system containing cells of different states. A method for synchronous recording of Cai change and single channel activity is also described. In cultured mammary epithelial cells, these methods revealed a propagating Cai signal induced by mechanical stimulation and spontaneous Cai oscillation with synchronous activation of calcium-activated potassium channels.

摘要

一种对fura - 2荧光进行连续图像减法的方法能够观察到细胞内钙（Cai）的实时（视频速率）变化。这种简单方法对于在含有不同状态细胞的系统中进行电生理同步测量非常有用。还描述了一种同步记录Cai变化和单通道活性的方法。在培养的乳腺上皮细胞中，这些方法揭示了由机械刺激诱导的传播性Cai信号以及伴随着钙激活钾通道同步激活的自发Cai振荡。

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