Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Foodborne Pathog Dis. 2012 Sep;9(9):792-5. doi: 10.1089/fpd.2012.1156. Epub 2012 Aug 14.
A total of 52 Shiga toxin-producing Escherichia coli (STEC) strains, isolated from fecal samples of six ibex, 12 chamois, 15 roe deer, and 19 red deer were further characterized by subtyping the stx genes, examining strains for the top nine serogroups and testing for the presence of eae and ehxA. Eleven of the 52 strains belonged to one of the top nine STEC O groups (O26, O45, O91, O103, O111, O113, O121, O145, and O157). Eight STEC strains were of serogroup O145, two strains of serogroup O113, and one strain of serogroup O157. None of the strains harbored stx2a, stx2e, or stx2f. Stx2b (24 strains) and stx1c (21 strains) were the most frequently detected stx subtypes, occurring alone or in combination with another stx subtype. Eight strains harbored stx2g, five strains stx2d, three strains stx1a, two strains stx2c, and one strain stx1d. Stx2g and stx1d were detected in strains not harboring any other stx subtype. The eae and ehxA genes were detected in two and 24 STEC strains, respectively. Considering both, the serogroups and the virulence factors, the majority of the STEC strains isolated from red deer, roe deer, chamois, and ibex do not show the typical patterns of highly pathogenic STEC strains. To assess the potential pathogenicity of STEC for humans, strain isolation and characterization is therefore of central importance.
共从 6 只野山羊、12 只岩羚羊、15 只狍和 19 只马鹿的粪便样本中分离出 52 株产志贺毒素大肠杆菌（STEC），进一步通过stx 基因分型、检测 9 种主要血清群和检测 eae 和 ehxA 来对菌株进行特征描述。在这 52 株菌株中，有 11 株属于前 9 种 STEC O 群之一（O26、O45、O91、O103、O111、O113、O121、O145 和 O157）。8 株 STEC 菌株属于 O145 血清群，2 株属于 O113 血清群，1 株属于 O157 血清群。没有菌株携带 stx2a、stx2e 或 stx2f。stx2b（24 株）和 stx1c（21 株）是最常检测到的 stx 亚型，单独存在或与另一种 stx 亚型一起存在。有 8 株携带 stx2g，5 株携带 stx2d，3 株携带 stx1a，2 株携带 stx2c，1 株携带 stx1d。stx2g 和 stx1d 存在于不携带任何其他 stx 亚型的菌株中。在 2 株和 24 株 STEC 菌株中分别检测到 eae 和 ehxA 基因。考虑到血清群和毒力因子，从马鹿、狍、岩羚羊和野山羊中分离出的大多数 STEC 菌株不具有典型的高致病性 STEC 菌株模式。为了评估 STEC 对人类的潜在致病性，因此，菌株分离和特征描述至关重要。
