Varcasia Bianca Maria, Tomassetti Francesco, De Santis Laura, Di Giamberardino Fabiola, Lovari Sarah, Bilei Stefano, De Santis Paola
Istituto Zooprofilattico Sperimentale Lazio e Toscana, "M. Aleandri", 00178 Rome, Italy.
Microorganisms. 2018 Dec 6;6(4):126. doi: 10.3390/microorganisms6040126.
The aim of this study was to determine the prevalence of Shiga toxin-producing in fresh beef marketed in 2017 in 13 regions of Italy, to evaluate the potential risk to human health. According to the ISO/TS 13136:2012 standard, 239 samples were analysed and nine were STEC positive, from which 20 strains were isolated. The STEC-positive samples were obtained from Calabria ( = 1), Campania ( = 1), Lazio ( = 2), Liguria ( = 1), Lombardia ( = 1) and Veneto ( = 3). All STEC strains were analysed for serogroups O26, O45, O55, O91, O103, O104, O111, O113, O121, O128, O145, O146 and O157, using Real-Time PCR. Three serogroups were identified amongst the 20 strains: O91 ( = 5), O113 ( = 2), and O157 ( = 1); the O-group for each of the 12 remaining STEC strains was not identified. Six subtypes were detected: , , , , and . Subtype was the most common, followed by and . Subtype was identified in only one -negative strain and occurred in combination with , and . The presence in meat of STEC strains being potentially harmful to human health shows the importance, during harvest, of implementing additional measures to reduce contamination risk.
本研究的目的是确定2017年在意大利13个地区销售的新鲜牛肉中产志贺毒素大肠杆菌的流行情况，以评估对人类健康的潜在风险。根据ISO/TS 13136:2012标准，分析了239个样本，其中9个为产志贺毒素大肠杆菌阳性，从中分离出20株菌株。产志贺毒素大肠杆菌阳性样本来自卡拉布里亚（=1）、坎帕尼亚（=1）、拉齐奥（=2）、利古里亚（=1）、伦巴第（=1）和威尼托（=3）。使用实时聚合酶链反应对所有产志贺毒素大肠杆菌菌株进行血清群O26、O45、O55、O91、O103、O104、O111、O113、O121、O128、O145、O146和O157分析。在20株菌株中鉴定出三个血清群：O91（=5）、O113（=2）和O157（=1）；其余12株产志贺毒素大肠杆菌菌株的O群未鉴定。检测到六种亚型：、、、、和。亚型最常见，其次是和。亚型仅在一株阴性菌株中鉴定出，并与、和同时出现。对人类健康有潜在危害的产志贺毒素大肠杆菌菌株存在于肉类中，这表明在收获期间实施额外措施以降低污染风险的重要性。
