State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Nov;97:737-40. doi: 10.1016/j.saa.2012.06.051. Epub 2012 Jul 10.
Based on the selective interaction between Hg(2+) ions and cyanuric acid (CA) and the anti-aggregation of CA stabilized gold nanoparticles (CA-Au NPs), a simple colorimetric method was developed for detecting Hg(2+) ions. In a medium of pH 7.4 tris-HCl buffer containing 8×10(-3) M NaCl, the CA-Au NPs solution was red, which was due to CA adsorbed onto the surface of Au NPs, stabilizing Au NPs against aggregation. When CA-Hg(II)-CA complex was formed in the presence of Hg(2+), the stability of CA-Au NPs reduced, and then aggregation of Au NPs occurred. Consequently, the color of the solution changed from red to blue and could easily be measured with a common spectrophotometer. The aggregation of Au NPs was also validated using transmission electron microscopy (TEM). The controlled experiment showed that other ions including Ba(2+), Ca(2+), Zn(2+), Cd(2+), Co(2+), Mn(2+), Cu(2+), Mg(2+), and Ni(2+) ions did not induce any distinct spectral changes, which constituted a Hg(2+)-selective sensor. A dynamic range of 1.6-16×10(-6)M Hg(2+) ions was observed at the optimized reaction condition. This method provides a potentially useful tool for Hg(2+) detection.
基于 Hg(2+) 离子与三聚氰胺（CA）的选择性相互作用以及 CA 稳定的金纳米粒子（CA-Au NPs）的抗聚集作用，开发了一种简单的比色法来检测 Hg(2+) 离子。在 pH 7.4 的 tris-HCl 缓冲液中，当存在 Hg(2+) 时，形成 CA-Hg(II)-CA 络合物，CA-Au NPs 的稳定性降低，Au NPs 发生聚集。因此，溶液的颜色从红色变为蓝色，并且可以使用普通分光光度计轻松测量。Au NPs 的聚集也使用透射电子显微镜（TEM）进行了验证。对照实验表明，包括 Ba(2+)、Ca(2+)、Zn(2+)、Cd(2+)、Co(2+)、Mn(2+)、Cu(2+)、Mg(2+)和 Ni(2+)在内的其他离子不会引起任何明显的光谱变化，这构成了一种 Hg(2+) 选择性传感器。在优化的反应条件下，观察到 1.6-16×10(-6)M Hg(2+) 离子的动态范围。该方法为 Hg(2+) 的检测提供了一种潜在有用的工具。
