Miot Hélio Amante, Brianezi Gabrielli, Tamega Andréia de Almeida, Miot Luciane Donida Bartoli
Department of Dermatology and Radiotherapy, School of Medicine of Botucatu, Universidade Estadual Paulista Julio de Mesquita Filho, Sao Paulo, Brazil.
An Bras Dermatol. 2012 Jul-Aug;87(4):608-11. doi: 10.1590/s0365-05962012000400014.
Morphometric analysis of tissue melanin may quantitatively contribute to research on pigmentation disorders. The authors present three methods for image analysis, which allow for identification of melanin-equivalent pixels in the epidermis using Fontana-Masson stain and, therefore, for the calculation of its percentage in the different epidermal layers. Moreover, they discuss the main elements related to the analysis and the need for rigorous standardization of the process.
组织黑色素的形态计量分析可能会对色素沉着紊乱的研究做出定量贡献。作者提出了三种图像分析方法，这些方法可以使用Fontana-Masson染色法识别表皮中黑色素等效像素，从而计算其在不同表皮层中的百分比。此外,他们还讨论了与分析相关的主要因素以及该过程进行严格标准化的必要性。
