Cole Benjamin J, Chory Joanne
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
Methods Mol Biol. 2012;918:1-7. doi: 10.1007/978-1-61779-995-2_1.
Time-resolved hypocotyl length measurements in seedlings have the potential to greatly aid genetic studies looking at light, hormone, and circadian regulation of cell expansion. Recently, several computer-based tools have been developed to quantify hypocotyl length during photomorphogenesis and early seedling development. Here we detail a method for quantifying Arabidopsis seedling hypocotyls in an image-based assay, focusing on light-grown seedlings responding to shade conditions.
对幼苗进行时间分辨下胚轴长度测量，有潜力极大地辅助关于光、激素以及细胞扩张的昼夜节律调节的遗传学研究。最近，已经开发了几种基于计算机的工具，用于在光形态建成和幼苗早期发育过程中量化下胚轴长度。在这里，我们详细介绍一种在基于图像的分析中量化拟南芥幼苗下胚轴的方法，重点关注对遮荫条件作出反应的光照生长幼苗。
