Pieruschka Roland, Klimov Denis, Berry Joseph A, Osmond C Barry, Rascher Uwe, Kolber Zbigniew S
Forschungszentrum Jülich, IBG: 2 Plant Sciences, Jülich, Germany.
Methods Mol Biol. 2012;918:51-9. doi: 10.1007/978-1-61779-995-2_5.
The interaction of plants with their environment is very dynamic. Studying the underlying processes is important for understanding and modeling plant response to changing environmental conditions. Photosynthesis varies largely between different plants and at different locations within a canopy of a single plant. Thus, continuous and spatially distributed monitoring is necessary to assess the dynamic response of photosynthesis to the environment. Limited scale of observation with portable instrumentation makes it difficult to examine large numbers of plants under different environmental conditions. We report here on the application of a recently developed technique, laser-induced fluorescence transient (LIFT), for continuous remote measurement of photosynthetic efficiency of selected leaves at a distance of up to 50 m. The ability to make continuous, automatic, and remote measurements of photosynthetic efficiency of leaves with the LIFT provides a new approach for studying the interaction of plants with the environment and may become an important tool in phenotyping photosynthetic properties in field applications.
植物与其环境的相互作用非常动态。研究其潜在过程对于理解和模拟植物对不断变化的环境条件的反应至关重要。不同植物之间以及单株植物冠层内不同位置的光合作用差异很大。因此，需要进行连续且空间分布的监测，以评估光合作用对环境的动态响应。便携式仪器的观测尺度有限，使得在不同环境条件下检测大量植物变得困难。我们在此报告一种最近开发的技术——激光诱导荧光瞬态（LIFT）的应用，该技术可在距离达50米处对选定叶片的光合效率进行连续远程测量。利用LIFT对叶片光合效率进行连续、自动和远程测量的能力，为研究植物与环境的相互作用提供了一种新方法，并且可能成为田间应用中光合特性表型分析的重要工具。
