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电感耦合等离子体质谱法作为植物高通量分析的工具。

Inductively coupled plasma-mass spectrometry as a tool for high-throughput analysis of plants.

作者信息

Seravalli Javier

机构信息

Redox Biology Center and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.

出版信息

Methods Mol Biol. 2012;918:269-88. doi: 10.1007/978-1-61779-995-2_14.

Abstract

Ionomics is the study of the elemental composition of biological tissues. It complements knowledge acquired by metabolomics, proteomics, bioinformatics, and genomics in elucidating the physiological status of plants as well as the identification of genes involved in the transport and metabolism of individual elements and their interactions. Inductively coupled plasma-mass spectrometry (ICP-MS) technology provides a very sensitive method for the medium- and high-throughput elemental analysis of plant tissues. This chapter introduces the plant physiologist to the ICP-MS technique, a method for sample preparation and analysis.

摘要

离子组学是对生物组织元素组成的研究。它在阐明植物生理状态以及识别参与单个元素的运输、代谢及其相互作用的基因方面,补充了代谢组学、蛋白质组学、生物信息学和基因组学所获得的知识。电感耦合等离子体质谱(ICP-MS)技术为植物组织的中高通量元素分析提供了一种非常灵敏的方法。本章向植物生理学家介绍ICP-MS技术、样品制备方法和分析方法。

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