State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China.
Small. 2012 Nov 19;8(22):3456-64. doi: 10.1002/smll.201200601. Epub 2012 Aug 14.
Novel core-shell structured Fe3O4@LnPO4 (Ln=Eu, Tb, Er) multifunctional microspheres with a magnetic Fe3O4 core and a LnPO4 shell covered with spikes are synthesized for the first time through the combination of a homogeneous precipitation approach and an ion-exchange process. Their potential for selective capture, rapid separation, and easy mass spectrometry (MS) labeling of the phosphopeptides from complex proteolytic digests are evaluated. These affinity microspheres can improve the specificity for capture of the phosphopeptides, realize fast magnetic separation, enhance the MS detection signals, and directly identify phosphopeptides through 80 Da mass loss in the mass spectra. The synthesis strategy could become a general and effective technique for similar core-shell hierarchical structures.
首次通过均相沉淀法和离子交换过程的结合，合成了具有磁性 Fe3O4 核和 LnPO4 壳的新型核壳结构 Fe3O4@LnPO4（Ln=Eu、Tb、Er）多功能微米球，其表面覆盖着刺。评估了它们从复杂蛋白水解物中选择性捕获、快速分离和易于质谱（MS）标记磷酸肽的潜力。这些亲和微球可以提高对磷酸肽的捕获特异性，实现快速磁分离，增强 MS 检测信号，并通过质谱中 80 Da 的质量损失直接鉴定磷酸肽。该合成策略可能成为类似核壳分层结构的通用有效技术。
