Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, Nebraska 68198-5840, USA.
Invest Ophthalmol Vis Sci. 2012 Sep 12;53(10):6115-21. doi: 10.1167/iovs.12-9570.
An intracanalicular scaffold (Hydrus microstent) designed to reduce intraocular pressure as a glaucoma treatment was tested in human anterior segments to determine changes in outflow facility (C).
Human eyes with no history of ocular disease or surgeries were perfused within 49 hours of death. The anterior segments were isolated and connected to a perfusion system. Flow rates were measured at pressures of 10, 20, 30, and 40 mm Hg. The scaffold was inserted into Schlemm's canal of the experimental eye, while a control eye underwent a sham procedure. Flow rate measurements were repeated at the four pressure levels. Individual C values were computed by dividing the flow rate by its corresponding pressure, and by averaging the four individual C measurements. The change in C between control and experimental eyes was assessed by the ratio of the baseline and second C measurement. In two eyes, the placement of the scaffold was evaluated histologically.
After scaffold implantation in the experimental eyes, the average C increased significantly from baseline (n = 9, P < 0.05). Ratios of C at all pressure levels, except for 10 mm Hg, were significantly higher in experimental eyes (n = 9) than control eyes (P < 0.05, n = 7). Histologically, the scaffold dilated Schlemm's canal with no visible damage to the trabecular meshwork.
The Hydrus Microstent provided an effective way to increase outflow facility in human eyes ex vivo.
一种旨在降低眼内压的管内支架（Hydrus microstent）被设计用于治疗青光眼，已在人类眼前节中进行了测试，以确定流出道通畅度（C）的变化。
从死亡后 49 小时内的无眼部疾病或手术史的人眼采集标本。将眼前节分离并连接到灌注系统。在 10、20、30 和 40 mmHg 的压力下测量流量。将支架插入实验眼的施莱姆氏管内，而对照眼则进行假手术。在四个压力水平下重复测量流量。通过将流量除以相应的压力，并平均四个个体 C 测量值，计算 C 值。通过比较基线和第二次 C 测量值的比值来评估对照眼和实验眼之间 C 的变化。在两只眼中，通过组织学评估支架的放置位置。
在实验眼中植入支架后，平均 C 从基线显著增加（n = 9，P <0.05）。除 10 mmHg 外，在实验眼（n = 9）中，所有压力水平的 C 比值均显著高于对照眼（n = 7，P <0.05）。组织学上，支架扩张了施莱姆氏管，而小梁网没有可见的损伤。
Hydrus Microstent 为增加人类离体眼前节的流出道通畅度提供了一种有效的方法。
