University of Campinas, Pediatric Dentistry Department, Piracicaba Dental School, Av. Limeira, 901, Piracicaba, SP 13414-903, Brazil.
J Biomed Opt. 2012 Jul;17(7):075008. doi: 10.1117/1.JBO.17.7.075008.
We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber and divided by six groups (n=5): NT-no treatment; CHX-2% chlorhexidine; CHXE-2% chlorhexidine+17% EDTA; E-17% EDTA; SH5-5.25% NaOCl; SH5E-5.25% NaOCl+17% EDTA. The inorganic and organic content was analyzed by FT-Raman. μEDXRF examined calcium (Ca) and phosphorus (P) content as well as Ca/P ratio. Impressions of specimens were evaluated by SEM. Data were submitted to Kruskal-Wallis and Dunn tests (p<0.05). Differences were observed among groups for the 960 cm(-1) peak. Ca and P content differences were significant (SH5>NT=SH5E>CHX>E>CHXE). CHXE and E presented the highest Ca/P ratio values compared to the other groups (p<0.05). The SEM images in the EDTA-treated groups had the highest number of open tubules. Erosion in the tubules was observed in CHX and SH5E groups. Endodontic agents change the inorganic and organic content of pulp chamber dentin. NaOCl used alone, or in association with EDTA, was the most effective agent considering chemical and morphological approaches.
我们通过扫描电子显微镜（SEM）、傅里叶变换拉曼光谱（FT-Raman）和微能量色散 X 射线荧光光谱（μEDXRF）检查了牙髓腔牙本质在用根管治疗剂后的形态和化学变化。将 30 颗牙齿切成暴露牙髓腔的切片，并分为六组（n=5）：NT-无处理；CHX-2%洗必泰；CHXE-2%洗必泰+17%EDTA；E-17%EDTA；SH5-5.25%次氯酸钠；SH5E-5.25%次氯酸钠+17%EDTA。FT-Raman 分析无机和有机含量。μEDXRF 检查钙（Ca）和磷（P）含量以及 Ca/P 比值。通过 SEM 对标本的印象进行评估。数据提交给 Kruskal-Wallis 和 Dunn 检验（p<0.05）。960cm(-1) 峰的组间存在差异。Ca 和 P 含量差异显著（SH5>NT=SH5E>CHX>E>CHXE）。与其他组相比，CHXE 和 E 具有最高的 Ca/P 比值（p<0.05）。EDTA 处理组的 SEM 图像中开放小管的数量最多。CHX 和 SH5E 组观察到小管的侵蚀。根管治疗剂改变了牙髓腔牙本质的无机和有机含量。单独使用次氯酸钠或与 EDTA 联合使用，从化学和形态学方法来看，是最有效的试剂。
