Osaka University, Graduate School of Engineering, Osaka 565-0871, Japan.
J Biomed Opt. 2012 Jul;17(7):078002. doi: 10.1117/1.JBO.17.7.078002.
Photodynamic therapy (PDT) is a photochemical modality approved for cancer treatment. PDT has demonstrated efficacy in early stage lung cancer and esophageal cancer. The accumulation of photosensitizers in cancer cells is necessary to enhance the therapeutic benefits of PDT; however, photosensitizers have low uptake efficiency. To overcome this limitation, a drug delivery system, such as the hemagglutinating virus of Japan envelope (HVJ-E) vector, is required. In this study, the combination of PDT and HVJ-E was investigated for enhancing the efficacy of PDT. The photosensitizers that were evaluated included 5-aminolaevulinic acid (5-ALA), protoporphyrin IX (PPIX), and HVJ-PPIX. The uptake of the photosensitizers as increased twenty-fold with the addition of HVJ-E. The cytotoxicity of conventional 5-ALA was enhanced by the addition of HVJ-E vector. In conclusion, HVJ-E vector improved the uptake of photosensitizers and the PDT effect.
光动力疗法（PDT）是一种已被批准用于癌症治疗的光化学疗法。PDT 已被证明对早期肺癌和食管癌有效。为了增强 PDT 的治疗效果，需要在癌细胞中积累光敏剂；然而，光敏剂的摄取效率很低。为了克服这一限制，需要使用药物递送系统，如血凝病毒日本包膜（HVJ-E）载体。在这项研究中，研究了 PDT 和 HVJ-E 的联合应用，以增强 PDT 的疗效。评估的光敏剂包括 5-氨基酮戊酸（5-ALA）、原卟啉 IX（PPIX）和 HVJ-PPIX。加入 HVJ-E 后，光敏剂的摄取增加了二十倍。加入 HVJ-E 载体后，常规 5-ALA 的细胞毒性增强。总之，HVJ-E 载体提高了光敏剂的摄取和 PDT 效果。
