Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Institute for Academic Initiatives, Osaka University, 1-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Photodiagnosis Photodyn Ther. 2014 Mar;11(1):48-54. doi: 10.1016/j.pdpdt.2013.10.001. Epub 2013 Oct 19.
In the clinic, it is often very difficult to treat drug-resistant advanced prostate cancer by conventional treatments. Photodynamic therapy (PDT) is a minimally invasive treatment that takes advantage of photochemical reactions between a photosensitizer and light. On the basis of several of its key characteristics, PDT is considered to be a promising novel method for treating drug-resistant prostate cancer.
For effective treatment of drug-resistant prostate cancer, we developed a novel agent termed porphyrus envelope, which was produced from PpIX lipid and hemagglutinating virus of Japan envelope (HVJ-E).
We inserted PpIX lipid into HVJ-E by centrifugation, and used the resultant porphyrus envelope in PDT of two drug-resistant prostate cancer cell lines, PC-3 and PC-3-DR.
Porphyrus envelope enhanced uptake of PpIX, and cytotoxicity of PDT, relative to free PpIX lipid or PpIX induced by 5-ALA.
PDT using porphyrus envelope has potential as a method for treating drug-resistant prostate cancer.
在临床上，通过常规治疗来治疗耐药性晚期前列腺癌往往非常困难。光动力疗法（PDT）是一种微创治疗方法，利用光敏剂和光之间的光化学反应。基于其几个关键特征，PDT 被认为是治疗耐药性前列腺癌的一种很有前途的新方法。
为了有效治疗耐药性前列腺癌，我们开发了一种新型试剂，称为卟啉包膜，它由 PpIX 脂质和日本血凝病毒包膜（HVJ-E）制成。
我们通过离心将 PpIX 脂质插入 HVJ-E 中，并将所得的卟啉包膜用于两种耐药性前列腺癌细胞系 PC-3 和 PC-3-DR 的 PDT 中。
与游离的 PpIX 脂质或 5-ALA 诱导的 PpIX 相比，卟啉包膜增强了 PpIX 的摄取和 PDT 的细胞毒性。
使用卟啉包膜的 PDT 有潜力成为治疗耐药性前列腺癌的一种方法。
