结核分枝杆菌吡嗪酰胺耐药常规验证的基因测序：pncA 而非 rpsA 的作用。
Gene sequencing for routine verification of pyrazinamide resistance in Mycobacterium tuberculosis: a role for pncA but not rpsA.
机构信息
Public Health Ontario, Public Health Laboratories, Toronto, Ontario, Canada.
出版信息
J Clin Microbiol. 2012 Nov;50(11):3726-8. doi: 10.1128/JCM.00620-12. Epub 2012 Aug 15.
Abstract
Pyrazinamide (PZA) is an important component of first-line therapy for the treatment of tuberculosis. Here, we evaluate targeted gene sequencing as a supplement to phenotypic PZA susceptibility testing of Mycobacterium tuberculosis. Routine sequencing of pncA, but not rpsA, is effective for verification of PZA susceptibility results.
摘要
吡嗪酰胺（PZA）是治疗结核病一线治疗方案中的重要组成部分。在这里，我们评估了靶向基因测序作为结核分枝杆菌表型 PZA 药敏试验的补充。常规测序 pncA 而不是 rpsA 可有效验证 PZA 药敏结果。
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本文引用的文献
1
Validation of pncA gene sequencing in combination with the mycobacterial growth indicator tube method to test susceptibility of Mycobacterium tuberculosis to pyrazinamide.验证结核分枝杆菌 pncA 基因测序联合分枝杆菌生长指示管法检测结核分枝杆菌对吡嗪酰胺的敏感性。
J Clin Microbiol. 2012 Feb;50(2):428-34. doi: 10.1128/JCM.05435-11. Epub 2011 Nov 16.
2
Pyrazinamide inhibits trans-translation in Mycobacterium tuberculosis.吡嗪酰胺抑制结核分枝杆菌中的转译-转译。
Science. 2011 Sep 16;333(6049):1630-2. doi: 10.1126/science.1208813. Epub 2011 Aug 11.
3
Pyrazinamide susceptibility testing in Mycobacterium tuberculosis: a systematic review with meta-analyses.结核分枝杆菌吡嗪酰胺药敏试验：系统评价与荟萃分析。
Antimicrob Agents Chemother. 2011 Oct;55(10):4499-505. doi: 10.1128/AAC.00630-11. Epub 2011 Jul 18.
4
Potential for erroneous results indicating resistance when using the Bactec MGIT 960 system for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide.使用 Bactec MGIT 960 系统检测结核分枝杆菌对吡嗪酰胺的敏感性时，可能会出现错误的耐药结果。
J Clin Microbiol. 2010 Jan;48(1):300-1. doi: 10.1128/JCM.01775-09. Epub 2009 Nov 18.
5
In-depth molecular characterization of Mycobacterium tuberculosis from New Delhi--predominance of drug resistant isolates of the 'modern' (TbD1) type.来自新德里的结核分枝杆菌的深入分子特征——“现代”（TbD1）型耐药菌株占主导地位
PLoS One. 2009;4(2):e4540. doi: 10.1371/journal.pone.0004540. Epub 2009 Feb 23.
6
Sequencing of the pncA gene in members of the Mycobacterium tuberculosis complex has important diagnostic applications: Identification of a species-specific pncA mutation in "Mycobacterium canettii" and the reliable and rapid predictor of pyrazinamide resistance.结核分枝杆菌复合群成员中pncA基因的测序具有重要的诊断应用：鉴定“堪萨斯分枝杆菌”中物种特异性的pncA突变以及吡嗪酰胺耐药性的可靠快速预测指标。
J Clin Microbiol. 2007 Feb;45(2):595-9. doi: 10.1128/JCM.01454-06. Epub 2006 Nov 29.
7
Widespread pyrazinamide-resistant Mycobacterium tuberculosis family in a low-incidence setting.低发病率地区广泛存在的耐吡嗪酰胺结核分枝杆菌家族。
J Clin Microbiol. 2003 Jul;41(7):2878-83. doi: 10.1128/JCM.41.7.2878-2883.2003.
8
The pncA gene from naturally pyrazinamide-resistant Mycobacterium avium encodes pyrazinamidase and confers pyrazinamide susceptibility to resistant M. tuberculosis complex organisms.来自天然耐吡嗪酰胺的鸟分枝杆菌的pncA基因编码吡嗪酰胺酶，并赋予耐吡嗪酰胺的结核分枝杆菌复合群菌株对吡嗪酰胺的敏感性。
Microbiology (Reading). 1997 Oct;143 ( Pt 10):3367-3373. doi: 10.1099/00221287-143-10-3367.
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Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus.编码吡嗪酰胺酶/烟酰胺酶的基因pncA发生突变，会导致结核杆菌对抗结核药物吡嗪酰胺产生耐药性。
Nat Med. 1996 Jun;2(6):662-7. doi: 10.1038/nm0696-662.
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