Division of Vector-Borne Disease, Centers for Disease Control and Prevention, Fort Collins, CO, USA.
ISME J. 2013 Jan;7(1):224-8. doi: 10.1038/ismej.2012.95. Epub 2012 Aug 16.
We collected Oropsylla montana from rock squirrels, Spermophilus varigatus, and infected a subset of collected fleas with Yersinia pestis, the etiological agent of plague. We used bar-tagged DNA pyrosequencing to characterize bacterial communities of wild, uninfected controls and infected fleas. Bacterial communities within Y. pestis-infected fleas were substantially more similar to one another than communities within wild or control fleas, suggesting that infection alters the bacterial community in a directed manner such that specific bacterial lineages are severely reduced in abundance or entirely eliminated from the community. Laboratory conditions also significantly altered flea-associated bacterial communities relative to wild communities, but much less so than Y. pestis infection. The abundance of Firmicutes decreased considerably in infected fleas, and Bacteroidetes were almost completely eliminated from both the control and infected fleas. Bartonella and Wolbachia were unaffected or responded positively to Y. pestis infection.
我们从岩松鼠和子午沙鼠收集了高山革螨，并将采集到的跳蚤的一部分感染了鼠疫耶尔森菌，鼠疫耶尔森菌是鼠疫的病原体。我们使用带有条形码的 DNA 焦磷酸测序来描述野生、未感染对照和感染跳蚤的细菌群落。感染鼠疫耶尔森菌的跳蚤内部的细菌群落彼此之间的相似性远远超过野生或对照跳蚤内部的细菌群落，这表明感染以定向的方式改变了细菌群落，使特定的细菌谱系在丰度上严重减少或从群落中完全消除。与野生群落相比，实验室条件也显著改变了跳蚤相关的细菌群落，但远不及鼠疫耶尔森菌感染的影响大。感染跳蚤中厚壁菌门的丰度显著降低，而拟杆菌门几乎从对照和感染的跳蚤中完全消除。巴尔通体和沃尔巴克氏体不受影响或对鼠疫耶尔森菌感染呈阳性反应。
