Department of Neurosurgery, Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hangzhou 310016, PR China.
Oncol Rep. 2012 Nov;28(5):1771-7. doi: 10.3892/or.2012.1970. Epub 2012 Aug 10.
Recent studies have revealed that miR-92a is overexpressed in several types of malignancies and provides a protumorigenic effect. Our findings demonstrate that the high expression of miR-92a in human glioma specimens is significantly correlated with low levels of BCL2L11 (Bim) protein and high-grade glioma. Here, we present the first evidence that miR-92a antisense oligonucleotide (AS-miR-92a) provides a tumor suppressive effect via induction of apoptosis in human glioma cells. In addition, we show that Bim is a direct functional target of miR-92a. Introducing Bim cDNA without 3'UTR abrogates miR-92a-induced cell survival. Further investigations will focus on the therapeutic use of AS-miR‑92a-mediated antitumor effects in glioma.
最近的研究表明，miR-92a 在几种类型的恶性肿瘤中过表达，并具有促进肿瘤发生的作用。我们的研究结果表明，miR-92a 在人胶质瘤标本中的高表达与 BCL2L11（Bim）蛋白水平降低和高级别胶质瘤显著相关。在这里，我们首次提供证据表明，miR-92a 反义寡核苷酸（AS-miR-92a）通过诱导人胶质瘤细胞凋亡发挥肿瘤抑制作用。此外，我们表明 Bim 是 miR-92a 的直接功能靶标。引入没有 3'UTR 的 Bim cDNA 可消除 miR-92a 诱导的细胞存活。进一步的研究将集中于 AS-miR-92a 在胶质瘤中的抗肿瘤作用的治疗用途。
