Department of Neurology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, PR China.
Mol Med Rep. 2012 Nov;6(5):1081-6. doi: 10.3892/mmr.2012.1025. Epub 2012 Aug 8.
The present study aimed to compare the 2-dimensional (2D) electrophoresis pattern of the cerebrospinal fluid (CSF) in multiple sclerosis (MS), neuromyelitis optica (NMO) and control individuals, to identify the proteins with differential expression and to examine their significance. CSF samples from the three groups were collected and total protein was isolated and quantified using the Bradford method. 2D electrophoresis of the samples was conducted using equal amounts of CSF. In the CSF 2D gel electrophoresis map, 118 points were obtained from the MS group, 155 points from the NMO group and 350 points from the normal control group. Non-matching proteins appeared in 14 spots in the MS group and in 45 spots in the NMO group, and were also expressed in the 2D electrophoresis pattern of the normal control group. Four differential proteins were identified through the HD-MS/MS and MASCOT network search. Pre-albumin (PA) was found only in the CSF 2D gel electrophoresis map of the MS patients. Keratin 1 was expressed in the normal control group, but not in the MS group. The difference in the expression of keratin 9 in the MS group was twice that in the normal control group. The expression of keratin 1, keratin 9 and transferrin in the NMO group was twice that in the normal control group. The expression of PA was found only in the CSF 2D gel electrophoresis map of the MS patients, and not in the NMO group. Keratin 1 was expressed in the NMO group, but not in the MS group. The expression of a variety of proteins in the 2D electrophoresis pattern of CSF was significantly different in the MS, NMO and control groups. PA, keratin 1, transferrin and keratin 9 are identified as significantly differentially expressed proteins.
本研究旨在比较多发性硬化症（MS）、视神经脊髓炎（NMO）和对照组患者的脑脊液（CSF）2 维电泳图谱，鉴定差异表达蛋白，并探讨其意义。收集三组 CSF 标本，采用 Bradford 法分离和定量总蛋白。用等量 CSF 进行 2D 电泳。在 CSF 2D 凝胶电泳图谱中，MS 组获得 118 个点，NMO 组获得 155 个点，正常对照组获得 350 个点。MS 组有 14 个点、NMO 组有 45 个点出现非匹配蛋白，且在正常对照组 2D 电泳图谱中也有表达。通过 HD-MS/MS 和 MASCOT 网络搜索鉴定出 4 种差异蛋白。前白蛋白（PA）仅在 MS 患者的 CSF 2D 凝胶电泳图谱中出现。角蛋白 1在正常对照组中表达，但在 MS 组中不表达。MS 组角蛋白 9 的表达差异是正常对照组的两倍。角蛋白 1、角蛋白 9 和转铁蛋白在 NMO 组的表达是正常对照组的两倍。PA 仅在 MS 患者的 CSF 2D 凝胶电泳图谱中出现，而在 NMO 组中未出现。角蛋白 1在 NMO 组中表达，但在 MS 组中不表达。MS、NMO 和对照组 CSF 2D 电泳图谱中多种蛋白的表达差异显著。PA、角蛋白 1、转铁蛋白和角蛋白 9 被鉴定为差异显著表达蛋白。
