Wegner Lars H
Karlsruhe Institute of Technology, Institute of Botany I and Institute of Pulsed Power and Microwave Technology, Eggenstein-Leopoldshafen, Germany.
Methods Mol Biol. 2012;913:35-66. doi: 10.1007/978-1-61779-986-0_3.
By insertion into an individual xylem vessel at the root base, the multifunctional xylem probe allows the monitoring of the xylem pressure, the radial electrical gradients in the root (the so-called trans-root potential, TRP), as well as the activity of a particular ion such as K(+) in the xylem sap of intact, transpiring plants. The biophysical and physiological significance of these parameters with respect to salt stress is briefly explained, and the assembly of the probe, the setup used for these measurements, and the experimental procedure are outlined in detail.
通过插入根部基部的单个木质部导管，多功能木质部探针可以监测木质部压力、根部的径向电势梯度（即所谓的跨根电势，TRP），以及完整的、正在蒸腾的植物木质部汁液中特定离子（如K(+)）的活性。简要解释了这些参数在盐胁迫方面的生物物理和生理意义，并详细概述了探针的组装、用于这些测量的设置以及实验步骤。
