在非肝细胞中重建整个丙型肝炎病毒生命周期。
Reconstitution of the entire hepatitis C virus life cycle in nonhepatic cells.
机构信息
INSERM, U748, Strasbourg, France.
出版信息
J Virol. 2012 Nov;86(21):11919-25. doi: 10.1128/JVI.01066-12. Epub 2012 Aug 15.
Abstract
Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.
摘要
丙型肝炎病毒（HCV）是一种嗜肝人类病毒，但对于限制 HCV 感染肝细胞的相关宿主因素，目前仅部分了解。我们证明，外源性表达特定的宿主因子可在人源非肝细胞 293T 细胞中重建完整的 HCV 生命周期。本研究表明 HCV 在人源非肝细胞中具有强大的进入、RNA 复制和产生感染性病毒的能力，并突出了 HCV 嗜肝性所需的关键宿主因子。
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