Liu Jinhui, He Ji, Chen Shu, Qin Fei, Wang Fang, Xu Gang, Zhu Faming, Lv Hangjun, Yan Lixing
Blood Center of Zhejiang Province, Zhejiang, People's Republic of China.
Transfus Med Hemother. 2012 Feb;39(1):23-27. doi: 10.1159/000335720. Epub 2011 Dec 27.
Umbilical cord blood (UCB) has successfully used for transplantation to treat hematologic malignancies and genetic diseases. Herein, we describe the experience generated in a single public UCB bank at Zhejiang Province in China. METHODS: Good manufacturing practice and standard operating procedures were used to address donor selection as well as UCB collection, processing, and cryopreservation. Total nucleated cells (TNCs), cellular viability, CD34+ cells, and colony-forming units were determined, and infectious diseases screening test, sterility test, and HLA typing for UCB units were done. RESULTS: Only 18.51% of all collected UCB units met storage criteria, and 7,056 UCB units were cryopreserved in 10 years. The volume of UCB units was 95.0 ± 22.0 ml. The number of TNCs before and after processing was 13.32 ± 3.63 × 10(8) and 10.63 ± 2.80 × 10(8), respectively, and the recovery rate was 80.71 ± 11.26%. 0.4344 ± 0.1874% of the TNCs were CD34+ cells. The CFU-GM was 32.1 ± 28.0 colonies per 1 × 10(5) nucleated cells. Based mainly on HLA and nucleated cell content, 26 UCB units were released for transplantation. CONCLUSIONS: A public UCB bank was successfully established in China; collection and processing of UCB units should be optimized in order to gain maximum volume and cell count.
脐带血已成功用于移植治疗血液系统恶性肿瘤和遗传性疾病。在此，我们描述了中国浙江省一家公共脐带血库的经验。
采用良好生产规范和标准操作程序进行供体选择以及脐带血的采集、处理和冷冻保存。测定了总核细胞（TNCs）、细胞活力、CD34+细胞和集落形成单位，并对脐带血单位进行了传染病筛查试验、无菌试验和HLA分型。
所有采集的脐带血单位中只有18.51%符合储存标准，10年内共冷冻保存了7056个脐带血单位。脐带血单位的体积为95.0±22.0 ml。处理前后TNCs的数量分别为13.32±3.63×10⁸和10.63±2.80×10⁸，回收率为80.71±11.26%。TNCs中0.4344±0.1874%为CD34+细胞。每1×10⁵个有核细胞的CFU-GM为32.1±28.0个集落。主要基于HLA和有核细胞含量，26个脐带血单位被放行用于移植。
中国成功建立了一家公共脐带血库；应优化脐带血单位的采集和处理，以获得最大体积和细胞数量。
