Hu P, Lai R, Xie D
Institute of Otology, Department of Otolaryngology and Head and Neck surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
B-ENT. 2012;8(2):77-84.
OBJECTIVES/HYPOTHESIS: Aminoglycosides may decrease the expression of some proteins participating in ion-exchange in the cochlear lateral wall. Connexin 26 expression in the lateral wall may play a role in acquired hearing loss by maintaining the endocochlear potential and potassium concentration in the endolymph. We examined the effects of gentamicin on the expression of connexin 26 to obtain a better understanding of aminoglycoside ototoxicity.
We detected changes in connexin 26 protein and mRNA expression in the cochlear lateral wall using immunohistochemistry staining, western blotting, and real-time PCR in rats after administration of a single dose of gentamicin.
The expression of connexin 26 increased over time in type III fibrocytes after gentamicin administration. Elevated protein levels were detected 3 h after the single injection of gentamicin; while, mRNA levels increased after 24 h.
Connexin 26 plays an important role in the acute effects of high-dose gentamicin and is probably involved in the pathogenesis of ototoxic deafness.
目的/假设：氨基糖苷类药物可能会降低参与耳蜗外侧壁离子交换的某些蛋白质的表达。外侧壁中连接蛋白26的表达可能通过维持内淋巴电位和内淋巴中的钾浓度在获得性听力损失中发挥作用。我们研究了庆大霉素对连接蛋白26表达的影响，以更好地理解氨基糖苷类药物的耳毒性。
我们在给大鼠单次注射庆大霉素后，使用免疫组织化学染色、蛋白质印迹法和实时聚合酶链反应检测耳蜗外侧壁中连接蛋白26蛋白和信使核糖核酸表达的变化。
庆大霉素给药后，III型纤维细胞中连接蛋白26的表达随时间增加。单次注射庆大霉素3小时后检测到蛋白质水平升高；而信使核糖核酸水平在24小时后增加。
连接蛋白26在高剂量庆大霉素的急性效应中起重要作用，可能参与耳毒性耳聋的发病机制。
