Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd., Fort Collins, CO 80521, USA.
J Med Entomol. 2012 Jul;49(4):939-41. doi: 10.1603/me11287.
A naturally occurring mutation was detected within the probe binding region targeting the envelope gene sequence of West Nile virus used in real-time polymerase chain reaction assays to test mosquito pools and other samples. A single C-->T transition 6nt from the 5' end of the 16mer in the envelope gene probe-binding region at genomic position 1,194 reduced assay sensitivity. The mutation first was detected in 2009 and persisted at a low prevalence into 2011. The mutation caused a 0.4% false negative error rate during 2011. These data emphasized the importance of confirmational testing and redundancy in surveillance systems relying on highly specific nucleic acid detection platforms.
在实时聚合酶链反应检测中，检测到针对西尼罗河病毒包膜基因序列的探针结合区域的一个自然发生的突变，该检测用于检测蚊子池和其他样本。在基因组位置 1194 处，包膜基因探针结合区域中的 16 碱基探针的 5'端第 6 位核苷酸由 C 到 T 的转换降低了检测的灵敏度。该突变于 2009 年首次被发现，并在 2011 年持续以低流行率存在。该突变在 2011 年导致了 0.4%的假阴性错误率。这些数据强调了在依赖高度特异性核酸检测平台的监测系统中，确认性测试和冗余的重要性。
