Wang Zhongwei, Lu Xin, Liang Yubo, Zheng Zheng
Fudan University, Shanghai, China.
J Aquat Anim Health. 2012 Sep;24(3):161-4. doi: 10.1080/08997659.2012.675926.
Species of the genus Marteilia (Phylum Paramyxea) are protozoan parasites of marine mollusks. Marteilia spp. have been detected in mollusks from different parts of the world, but the presence of these parasites in China has not been previously reported. Therefore, a survey was conducted to look for the presence of Marteilia spp. in blue mussels Mytilus edulis and Asian green mussels Perna viridis collected along China's coasts. Histological and PCR analyses revealed that 5 of 180 M. edulis (prevalence = 2.8%) were positive for infection with a Marteilia-like organism, whereas the parasite was not detected in any of the 80 P. viridis individuals tested. Total genomic DNA was extracted from the infected tissue sections for PCR amplification. The PCR amplification with Marteilia primers SS1 and SAS1 yielded the expected 641-bp product. Sequencing results showed that the 18S ribosomal RNA gene fragment from the protozoans found in M. edulis from China was 88% similar to that of Marteilia refringens, a species that was reported from M. edulis and European flat oysters Ostrea edulis collected in France. This is the first report of a Marteilia-like organism infecting M. edulis in China.
马尔太虫属（副黏菌门）的物种是海洋软体动物的原生动物寄生虫。马尔太虫属物种已在世界不同地区的软体动物中被检测到，但此前在中国尚未报道过这些寄生虫的存在。因此，开展了一项调查，以寻找在中国沿海采集的蓝贻贝（Mytilus edulis）和翡翠贻贝（Perna viridis）中马尔太虫属物种的存在情况。组织学和PCR分析显示，180只蓝贻贝中有5只（感染率=2.8%）感染了一种类似马尔太虫的生物呈阳性，而在测试的80只翡翠贻贝个体中均未检测到该寄生虫。从感染的组织切片中提取总基因组DNA用于PCR扩增。用马尔太虫引物SS1和SAS1进行PCR扩增得到了预期的641bp产物。测序结果表明，在中国蓝贻贝中发现的原生动物的18S核糖体RNA基因片段与马尔太虫（Marteilia refringens）的该片段相似度为88%，马尔太虫是在法国采集的蓝贻贝和欧洲扁牡蛎（Ostrea edulis）中报道的一个物种。这是中国首次报道一种类似马尔太虫的生物感染蓝贻贝。
