McTighe Shane P, Vaidyanathan Rajeev
SRI International, 140 Research Drive, Harrisonburg, VA 22802, USA.
Vector Borne Zoonotic Dis. 2012 Oct;12(10):867-71. doi: 10.1089/vbz.2012.0978. Epub 2012 Aug 16.
We evaluated recently colonized samples from Virginia and Georgia (USA) of Aedes albopictus, an important vector of chikungunya virus (CHIKV), to determine whether they became infected with and transmitted COM125, a CHIKV isolate from the Comoros Islands. Seven days after imbibing an infective blood meal containing a minimum of 8.5×10(4) plaque-forming units (pfu)/mL, 31-86% of A. albopictus from the counties of Fairfax, Loudon, Rockingham, and Suffolk, Virginia, and Fulton, Georgia were infected. The average viral titer per mosquito was 1.1×10(4) pfu/mL (2×10(2)-3.3×10(4)). We detected CHIKV in salivary expectorate of infected mosquitoes from Rockingham (8%), Fulton (22%), and Loudon (48%) counties 7 days after blood feeding. Because CHIKV has no vaccines or specific antiviral treatments, vector control and education are critical to prevent its transmission. We discuss how local populations of A. albopictus could transmit CHIKV introduced to the southeastern USA from the Indian Ocean or Indian Subcontinent.
我们评估了近期从美国弗吉尼亚州和佐治亚州采集的白纹伊蚊样本，白纹伊蚊是基孔肯雅病毒（CHIKV）的重要传播媒介，目的是确定它们是否感染并传播了来自科摩罗群岛的CHIKV分离株COM125。在吸食含有至少8.5×10⁴ 蚀斑形成单位（pfu）/mL的感染性血餐7天后，来自弗吉尼亚州费尔法克斯、劳登、罗金厄姆和萨福克县以及佐治亚州富尔顿县的31%-86%的白纹伊蚊被感染。每只蚊子的平均病毒滴度为1.1×10⁴ pfu/mL（2×10² - 3.3×10⁴）。在吸血7天后，我们在来自罗金厄姆县（8%）、富尔顿县（22%）和劳登县（48%）的受感染蚊子的唾液中检测到了CHIKV。由于CHIKV没有疫苗或特定的抗病毒治疗方法，病媒控制和教育对于预防其传播至关重要。我们讨论了当地白纹伊蚊种群如何传播从印度洋或印度次大陆引入美国东南部的CHIKV。
