Centro de Referencia para Lactobacilos, CERELA-CONICET, Tucumán, Argentina.
J Appl Microbiol. 2012 Dec;113(6):1517-29. doi: 10.1111/j.1365-2672.2012.05429.x. Epub 2012 Sep 7.
To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains.
Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet staining. Only Lactobacillus rhamnosus Centro de Referencia para Lactobacilos Culture Collection (CRL) 1332, Lact. reuteri CRL 1324 and Lact. delbrueckii CRL 1510 were able to grow and form biofilm in culture media without Tween 80. However, Lact. gasseri CRL 1263 (a non-biofilm-forming strain) did not grow in these media. Scanning electron microscopy showed that Lact. rhamnosus CRL 1332 and Lact. reuteri CRL 1324 formed a highly structured biofilm, but only Lact. reuteri CRL 1324 showed a high amount of extracellular material in medium without Tween.
Biofilm formation was significantly influenced by the strain, culture medium, inoculum concentration, microbial growth and chemical nature of the support used for the assay.
The results allow the selection of biofilm-forming vaginal Lactobacillus strains and the conditions and factors that affect this phenomenon.
评估阴道乳杆菌在不同培养条件下形成生物膜的能力，并确定所选菌株的生长与生物膜形成能力之间的关系。
通过结晶紫染色法对 15 株来自人阴道的乳杆菌进行生物膜形成试验。只有鼠李糖乳杆菌 Centro de Referencia para Lactobacilos 培养物收集中心（CRL）1332、雷氏乳杆菌 CRL 1324 和德氏乳杆菌 CRL 1510 能够在不含吐温 80 的培养基中生长并形成生物膜。然而，格氏乳杆菌 CRL 1263（一种非生物膜形成菌株）不能在这些培养基中生长。扫描电子显微镜显示，鼠李糖乳杆菌 CRL 1332 和雷氏乳杆菌 CRL 1324 形成了高度结构化的生物膜，但只有雷氏乳杆菌 CRL 1324 在不含吐温的培养基中显示出大量的细胞外物质。
生物膜形成受到菌株、培养基、接种物浓度、微生物生长和用于测定的支持物的化学性质的显著影响。
这些结果允许选择形成生物膜的阴道乳杆菌菌株以及影响这种现象的条件和因素。
