Institute of Photonic Technology, Jena, Germany.
Anal Chem. 2012 Sep 18;84(18):7845-51. doi: 10.1021/ac301522d. Epub 2012 Sep 7.
Visualization as well as characterization of inner arterial plaque depositions is of vital diagnostic interest, especially for the early recognition of vulnerable plaques. Established clinical techniques provide valuable visual information but cannot deliver information about the chemical composition of individual plaques. Here, we employ Raman-probe spectroscopy to characterize the plaque compositions of arterial walls on a rabbit model in vivo, using a miniaturized filtered probe with one excitation and 12 collection fibers integrated in a 1 mm sleeve. Rabbits were treated with a cholesterol-enriched diet. The methodology can improve the efficiency of animal experiments and shows great potential for applications in cardiovascular research. In order to further characterize the plaque depositions visually, coherent anti-Stokes Raman scattering (CARS) microscopy images have been acquired and are compared with the Raman-probe results.
可视化以及动脉斑块内部特征的描述对诊断具有重要意义，尤其是对于易损斑块的早期识别。现有的临床技术提供了有价值的可视化信息，但无法提供关于单个斑块化学成分的信息。在这里，我们使用拉曼探针光谱法，通过在 1 毫米套管中集成一个激发和 12 个收集光纤的微型过滤探针，对体内兔模型的动脉壁斑块成分进行了特征描述。兔子接受富含胆固醇的饮食。该方法可以提高动物实验的效率，并在心血管研究中具有广泛的应用潜力。为了进一步可视化地描述斑块沉积，我们获取了相干反斯托克斯拉曼散射（CARS）显微镜图像，并将其与拉曼探针结果进行了比较。
