Applied Microbiology Division, National Food Research Institute, Tsukuba, Ibaraki, Japan.
J Agric Food Chem. 2012 Sep 19;60(37):9519-27. doi: 10.1021/jf3020975. Epub 2012 Sep 7.
Type B trichothecenes, deoxynivalenol (DON) and nivalenol (NIV), are secondary metabolites of Fusarium species and are major pollutants in food and feed products. Recently, the production trend of their derivatives, 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), and 4-acetylnivalenol (4-AcNIV or fusarenon-X), has been changing in various regions worldwide. Although in vivo behavior has been reported, it is necessary to acquire more detailed information about these derivatives. Here, the yeast PDR5 mutant was used for toxicity evaluation, and the growth test revealed that DON, 15-AcDON, and 4-AcNIV had higher toxicity compared to 3-AcDON and NIV. 15-AcDON exerted the most significant gene expression changes, and cellular localization clustering exhibited repression of mitochondrial ribosomal genes. This study suggests that the toxicity trends of both DON products (DON and its derivatives) and NIV products (NIV and its derivatives) are similar to those observed in mammalian cells, with a notable toxic response to 15-AcDON.
B 型单端孢霉烯族化合物，脱氧雪腐镰刀菌烯醇（DON）和雪腐镰刀菌烯醇（NIV），是镰刀菌属的次生代谢产物，是食品和饲料产品中的主要污染物。最近，它们的衍生物，3-乙酰脱氧雪腐镰刀菌烯醇（3-AcDON）、15-乙酰脱氧雪腐镰刀菌烯醇（15-AcDON）和 4-乙酰雪腐镰刀菌烯醇（4-AcNIV 或 fusarenon-X）的产生趋势在世界范围内的不同地区都发生了变化。尽管已经报道了体内行为，但有必要获取这些衍生物更详细的信息。在这里，使用酵母 PDR5 突变体进行毒性评估，生长试验表明 DON、15-AcDON 和 4-AcNIV 的毒性比 3-AcDON 和 NIV 更高。15-AcDON 引起了最显著的基因表达变化，细胞定位聚类显示线粒体核糖体基因受到抑制。本研究表明，DON 产物（DON 及其衍生物）和 NIV 产物（NIV 及其衍生物）的毒性趋势与在哺乳动物细胞中观察到的相似，对 15-AcDON 有明显的毒性反应。
