Evain-Brion D, Alsat E, Mirlesse V, Dodeur M, Scippo M L, Hennen G, Frankenne F
Laboratoire de Physiopathologie du Développement, Ecole Normale Supérieure, Paris, France.
Horm Res. 1990;33(6):256-9. doi: 10.1159/000181530.
In this study, we have cultured in vitro purified trophoblastic cells from first-trimester and term human placenta. These cells were obtained by specific enzymatic digestion and centrifugation through a Percoll gradient. Using 2 specific monoclonal antibodies, the pituitary 22-kD growth hormone (GH) and the placental GH variant were assayed in the culture medium by radioimmunoassay. After 48 h of culture, only the placental GH variant was measured in the medium corresponding to first-trimester placenta (3.4 ng/24 h/10(5) cells). Surprisingly, an immunoactivity pattern of pituitary GH type was found in 3 out of 5 media conditioned with term placenta cells, while GH immunoactivity was very low, around the detection level, in the 2 others. These secretions are not modified with the time in culture and the state of differentiation of the cells from cytotrophoblast to syncytiotrophoblast. Neither in early nor in term placenta does the addition of GH-releasing factor (10(-6) M in the culture medium) stimulate the secretion of pituitary 22-kD GH or placental GH variant.
在本研究中，我们对来自孕早期和足月人胎盘的纯化滋养层细胞进行了体外培养。这些细胞通过特定的酶消化和经Percoll梯度离心获得。使用2种特异性单克隆抗体，通过放射免疫分析法对培养基中的垂体22-kD生长激素（GH）和胎盘生长激素变体进行测定。培养48小时后，在对应于孕早期胎盘的培养基中仅检测到胎盘生长激素变体（3.4 ng/24小时/10⁵个细胞）。令人惊讶的是，在用足月胎盘细胞处理的5种培养基中，有3种出现了垂体GH型的免疫活性模式，而另外2种培养基中的GH免疫活性非常低，接近检测水平。这些分泌产物在培养过程中不会随时间以及细胞从细胞滋养层向合体滋养层的分化状态而改变。无论是在早期胎盘还是足月胎盘中，添加生长激素释放因子（培养基中浓度为10⁻⁶ M）均不会刺激垂体22-kD GH或胎盘生长激素变体的分泌。
