Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia 38400-902, Minas Gerais, Brazil.
Acta Trop. 2012 Nov;124(2):166-9. doi: 10.1016/j.actatropica.2012.07.010. Epub 2012 Aug 7.
This study was performed in order to develop a novel approach based on antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid (BALF) samples. For that purpose Wistar rats immunosuppressed or not were experimentally infected with Strongyloides venezuelensis. The microtiter plates were coated with alkaline parasite extract for antibody detection and with IgG anti-S. venezuelensis for antigen and immune complex detection. The immune serum was able to detect 1.56 μg/mL of L3 antigens in BALF samples. ELISA sensitivity was 96.6%, 71.6% and 91.6% for antigen, antibody and immune complex, respectively, and the specificity was 100% for all methods. Antigen detection in BALF samples showed to be a good approach for evaluating the kinetics of infection in non immunosuppressed or immunosuppressed rats. IgG was detected in non immunosuppressed rats from day 8 p.i. and in immunosuppressed rats from day 2 p.i. Moreover, immune complex was detected during the entire kinetic for both groups. In conclusion, association of antigen, antibody and immune complex detection in BALF samples seems to be an alternative approach for early strongyloidiasis diagnosis particularly in immunosuppressed individuals.
本研究旨在开发一种新的方法，基于酶联免疫吸附试验（ELISA）检测支气管肺泡灌洗液（BALF）样本中的抗原、抗体和免疫复合物。为此，实验性地感染了委内瑞拉旋毛虫的免疫抑制或未免疫抑制的 Wistar 大鼠。微量滴定板用碱性寄生虫提取物包被，用于检测抗体，用 IgG 抗 S. venezuelensis 检测抗原和免疫复合物。免疫血清能够检测到 BALF 样本中 1.56μg/ml 的 L3 抗原。ELISA 的灵敏度分别为抗原、抗体和免疫复合物的 96.6%、71.6%和 91.6%，所有方法的特异性均为 100%。BALF 样本中抗原的检测似乎是评估非免疫抑制或免疫抑制大鼠感染动力学的一种很好的方法。非免疫抑制大鼠从感染后第 8 天开始检测到 IgG，免疫抑制大鼠从感染后第 2 天开始检测到 IgG。此外，两组的整个动力学过程中都检测到了免疫复合物。总之，BALF 样本中抗原、抗体和免疫复合物的联合检测似乎是早期旋毛虫病诊断的一种替代方法，特别是在免疫抑制个体中。
