Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia 38400-902, Minas Gerais, Brazil.
Exp Parasitol. 2012 Mar;130(3):205-8. doi: 10.1016/j.exppara.2012.01.007. Epub 2012 Jan 28.
In order to establish an antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in serum samples, normal or immunocompromised Wistar rats experimentally infected with Strongyloides venezuelensis were used. The microtitre plates were coated with IgG anti-S. venezuelensis for antigen and immune complex detection and with alkaline parasite extract for antibody detection. Analysis revealed at least 12.5 μg/mL of S. venezuelensis specific antigens in serum samples. Assay for antigen detection was not a good approach for evaluating infection in normal or immunocompromised rats. In normal rats IgG specific for S. venezuelensis was preferentially detected during the first 13 days post-infection (p.i.) and immune complex detection was significantly reduced in 21 p.i. day. On the other hand, in immunocompromised rats, IgG and immune complex were detected during the entire kinetic (5, 8, 13 and 21 p.i). These results suggest that immune complex screening seems to be an alternative for early strongyloidiasis diagnosis in immunocompromised individuals.
为了建立抗原、抗体和免疫复合物的酶联免疫吸附试验（ELISA）检测方法，本研究使用正常或免疫功能低下的实验感染委内瑞拉旋毛虫的 Wistar 大鼠。微量滴定板用 IgG 抗委内瑞拉旋毛虫包被，用于抗原和免疫复合物检测，用碱性寄生虫提取物检测抗体。分析显示，血清样本中至少有 12.5 μg/mL 的委内瑞拉旋毛虫特异性抗原。抗原检测方法不适用于评估正常或免疫功能低下大鼠的感染情况。在正常大鼠中，感染后第 13 天（p.i.）优先检测到针对 S. venezuelensis 的 IgG 特异性，而 21 p.i. 时免疫复合物检测显著减少。另一方面，在免疫功能低下的大鼠中，IgG 和免疫复合物在整个动力学过程中均被检测到（5、8、13 和 21 p.i.）。这些结果表明，免疫复合物筛查似乎是免疫功能低下个体早期诊断旋毛虫病的一种替代方法。
