Sakami Tomoko, Andoh Tadashi, Morita Tetsuo, Yamamoto Yoshihisa
Tohoku National Fisheries Research Institute, Fisheries Research Agency, 3-27-5, Shinhama-Cho, Shiogama, Miyagi 985-0001, Japan.
Mar Genomics. 2012 Sep;7:27-31. doi: 10.1016/j.margen.2012.04.006. Epub 2012 May 19.
We constructed ammonia monooxygenase alpha subunit (amoA) gene clone libraries of ammonia-oxidizing archaea (AOA) and bacteria (AOB) from three biofiltration tanks used for closed marine fish culture systems. The number of operational taxonomic units (OTUs) found in any one place was 76%-80% of the total OTUs in each tank for AOA and 100% for AOB when OUTs were defined on the basis of a 5% nucleotide difference. In a phylogenetic tree, all of the AOA amoA sequences fell into a cluster, which contained Candidatus Nitrosopumilus maritimus. All of the AOB amoA sequences were related to the Nitrosospira lineage. These results indicated that different ammonia oxidizers were present in different tanks, but that the dominant phylogenetic types were stable. In a biofiltration tank to which a high concentration of ammonium chloride was added periodically to condition the biofilter materials, most of the AOA amoA sequences were different from the dominant one observed in the fish culture tanks. The AOB amoA sequences were also different, and were similar to those of Nitrosomonas aestuarii. These findings suggest that high concentration ammonia loads have a considerable affect on ammonia-oxidizer community composition.
我们构建了来自用于封闭海水鱼类养殖系统的三个生物滤池的氨氧化古菌（AOA）和细菌（AOB）的氨单加氧酶α亚基（amoA）基因克隆文库。当基于5%的核苷酸差异定义操作分类单元（OTU）时，在任何一个地方发现的OTU数量，对于AOA而言是每个滤池中总OTU的76%-80%，对于AOB则为100%。在系统发育树中，所有AOA的amoA序列都落入一个簇中，该簇包含暂定名嗜盐亚硝化侏儒菌。所有AOB的amoA序列都与亚硝化螺菌谱系相关。这些结果表明，不同的氨氧化菌存在于不同的滤池中，但占主导地位的系统发育类型是稳定的。在一个定期添加高浓度氯化铵以调节生物滤料的生物滤池中，大多数AOA的amoA序列与在鱼类养殖滤池中观察到的优势序列不同。AOB的amoA序列也不同，并且与河口亚硝化单胞菌的序列相似。这些发现表明，高浓度氨负荷对氨氧化菌群落组成有相当大的影响。
