Wang Wenlan, Tian Liang, Li Ya, Wang Xiaojun, Xia Feng, Li Li, Li Jinsheng, Zhang Zuoming
Department of Clinical Aerospace Medicine, School of Aerospace Medicine, Fourth Military Medical University, Xi' an, Shaanxi Province, China.
J Emerg Med. 2013 Jan;44(1):107-15. doi: 10.1016/j.jemermed.2012.01.065. Epub 2012 Aug 14.
Studies have shown that inhalation of hydrogen gas, which acts as an antioxidant, can protect the brain against free radicals in rats with ischemia-reperfusion. The neuronal damage caused by acute carbon monoxide (CO) poisoning is partly free radical mediated. We hypothesize that hydrogen may prevent neurological damage from CO poisoning.
This study is designed to test whether hydrogen (H(2))-rich saline will have a protective effect on rats with acute CO poisoning.
Male Sprague-Dawley rats were subjected to CO poisoning. H(2)-rich saline was administered by peritoneal injection (6 mL/kg/24 h). We used the Morris water maze and the open field test to determine cognitive function. After cognitive function studies, rats were decapitated and the levels of trace elements copper (Cu), zinc (Zn), and iron (Fe) in serum and brain were assessed by flame atomic absorption spectrometry. Necrosis, apoptosis, and autophagy of neurons were assessed by H-E staining and immunohistochemical staining in another group of rats.
H(2)-rich saline treatment improved the cognitive deficits and reduced the degree of necrosis, apoptosis, and cell autophagy in rats. Additionally, H(2)-rich saline decreased the content of Fe in serum and brain in these rats, and increased the content of serum Cu related to free radical metabolism.
H(2)-rich saline may effectively protect the brain from injury after acute CO poisoning. The mechanism of this protection may be related to lessening oxidative damage by affecting trace elements in vivo.
研究表明，吸入具有抗氧化作用的氢气可保护缺血再灌注大鼠的大脑免受自由基损伤。急性一氧化碳（CO）中毒所致的神经元损伤部分由自由基介导。我们推测氢气可能预防CO中毒所致的神经损伤。
本研究旨在检测富氢盐水对急性CO中毒大鼠是否具有保护作用。
将雄性Sprague-Dawley大鼠进行CO中毒处理。通过腹腔注射给予富氢盐水（6 mL/kg/24 h）。我们使用Morris水迷宫和旷场试验来测定认知功能。在认知功能研究后，将大鼠断头，通过火焰原子吸收光谱法评估血清和脑中微量元素铜（Cu）、锌（Zn）和铁（Fe）的水平。在另一组大鼠中，通过苏木精-伊红染色和免疫组织化学染色评估神经元的坏死、凋亡和自噬情况。
富氢盐水治疗改善了大鼠的认知缺陷，降低了坏死、凋亡和细胞自噬的程度。此外，富氢盐水降低了这些大鼠血清和脑中Fe的含量，并增加了与自由基代谢相关的血清Cu的含量。
富氢盐水可能有效保护急性CO中毒后的大脑免受损伤。这种保护机制可能与通过影响体内微量元素减轻氧化损伤有关。
