Department of Animal Pathology, University of Turin, Italy.
Res Vet Sci. 2013 Feb;94(1):111-4. doi: 10.1016/j.rvsc.2012.07.017. Epub 2012 Aug 13.
Natural infections of cattle associated with Listeria innocua have not been reported. This report describes the first case of cerebral listeriosis in a bull due to Listeria innocua. The animal presented neurological signs characterized by weakness, incoordination and recumbency. Histopathologic evaluation of brain tissue revealed multifocal microabscesses, perivascular lymphocytic cuffing, vasculitis, oedema and haemorrhages. All lesions extended from the medulla oblongata to the basal nuclei/parietal cortex area. Indirect immunohistochemistry labelled for Listeria sp. in the brain tissue, but not for Listeria monocytogenes, neurotropic Flaviviruses, BVDV, bovine Herpesvirus 1, Chlamydophila spp. and Histophilus somni. PCR was negative for ovine herpesvirus. L. innocua was isolated from brainstem and identified by biochemical tests (Camp and beta-hemolysis negative). Subsequently, the species was confirmed by a duplex PCR and minisequencing assays. L. innocua should be histologically considered as a differential diagnosis of thrombotic meningoencephalitis, malignant catarrhal fever and cerebral listeriosis due to L. monocytogenes in cattle.
尚未有牛自然感染无害李斯特菌的报道。本报告描述了首例因无害李斯特菌引起的公牛脑部李斯特菌病。该动物表现出以虚弱、共济失调和倒地为特征的神经症状。脑组织的组织病理学评估显示多发性微脓肿、血管周围淋巴细胞袖套、血管炎、水肿和出血。所有病变从延髓延伸到基底核/顶叶皮质区。间接免疫组织化学标记了脑组织中的李斯特菌，但未标记李斯特菌单核细胞增多症、亲神经 flaviviruses、牛病毒性腹泻病毒、牛疱疹病毒 1、衣原体属和嗜血杆菌属。绵羊疱疹病毒的 PCR 为阴性。无害李斯特菌从脑干中分离出来，并通过生化试验（无 Camp 和β溶血）进行鉴定。随后，通过双重 PCR 和 mini 测序试验确认了该物种。在组织学上，无害李斯特菌应被视为牛血栓性脑膜脑炎、恶性卡他热和由李斯特菌单核细胞增多症引起的脑部李斯特菌病的鉴别诊断。
