Li Xue, Feng Yi, Pan Fang, Wang Xiao-ning
Department of Anesthesiology, Peking University People's Hospital, Beijing, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2012 Aug 18;44(4):612-6.
To investigate the effects of L-arginine (L-Arg) on acid-base balance and thromboelastograph (TEG) in rabbits after banked-blood transfusion.
Fifty New Zealand white rabbits, of which 20 were banked-blood donors, and the remaining 30 were randomly divided into 3 groups (n=10).
hypovolemic models were made through blood loss by 20% of blood volume, and were retransfused with equal volume of banked-blood (14 days) 30 min later. Normal saline 1.2 mL/kg was given intravenously 5 min before transfusion; Sham operation group: the rabbits only received 25% L-Arg 300 mg/kg (1.2 mL/kg) intravenously at the same time-point as control group, without blood loss and banked-blood transfusion; L-Arg group: the rabbits received the same procedure as control group,equal volume of L-Arg was given as the control instead of normal saline. Blood gas /electrolyte analyses were determined before (T0) and after blood loss (T1), immediately after banked-blood transfusion (T2), 60 min (T3) and 120 min (T4) after banked-blood transfusion. TEG were checked only at T0 and T4.
In control group and L-Arg group, pH values were declined after blood loss and banked-blood transfusion, and then increased after the first drop. The pH value of control group at T2 was significantly lower than that at T0 and T1 (P<0.05). Base excess (BE) values continued to decrease from T0 to T4 of the three groups, which were significantly lower at T4 than at T0 in sham operation group (P<0.05). As to the concentration of blood lactate (Lac),there were no significant differences between the three groups at T0. In sham operation group and L-Arg group, Lac decreased from T0 to T4 (P<0.05), which was significantly lower than that of control group at the same time-point (P<0.05). The TEG had no significant difference within and among the groups at T0 and T4 (P>0.05).
L-Arg 300 mg/kg intravenous injection can improve tissue perfusion in rabbits receiving banked-blood transfusion, while there was little effect on acid-base balance and TEG.
探讨L-精氨酸（L-Arg）对库存血输注后家兔酸碱平衡及血栓弹力图（TEG）的影响。
50只新西兰白兔，其中20只为库存血供血者，其余30只随机分为3组（n = 10）。
通过失血量为血容量的20%制成低血容量模型，30分钟后输注等量库存血（保存14天）。输血前5分钟静脉注射1.2 mL/kg生理盐水；假手术组：家兔在与对照组相同时间点仅静脉注射25% L-Arg 300 mg/kg（1.2 mL/kg），无失血及库存血输注；L-Arg组：家兔接受与对照组相同的操作，给予与对照组等量的L-Arg代替生理盐水。在失血前（T0）、失血后（T1）、库存血输注后即刻（T2）、库存血输注后60分钟（T3）和120分钟（T4）进行血气/电解质分析。仅在T0和T4检查TEG。
对照组和L-Arg组失血及库存血输注后pH值下降，首次下降后升高。对照组T2时pH值显著低于T0和T1时（P<0.05）。三组从T0至T4碱剩余（BE）值持续下降，假手术组T4时显著低于T0时（P<0.05）。血乳酸（Lac）浓度在T0时三组间无显著差异。假手术组和L-Arg组Lac从T0至T4下降（P<0.05），且在同一时间点显著低于对照组（P<0.05）。TEG在T0和T4时组内及组间无显著差异（P>0.05）。
静脉注射300 mg/kg L-Arg可改善接受库存血输注家兔的组织灌注，而对酸碱平衡及TEG影响较小。
