Richmond R E, De Angelo A B, Daniel F B, Lindahl R
Department of Biological Sciences, Northern Kentucky University, Highland Heights 41076.
Histochem J. 1990 Oct;22(10):526-9. doi: 10.1007/BF01005974.
This communication describes a method and results for the immunohistochemical detection of a tumour-associated isoenzyme of aldehyde dehydrogenase (BALDH). The method is a substantial improvement over standard histochemical detection methods which require either frozen or mildly fixed tissues, since BALDH expression was detected in the cells of formalin-fixed paraffin-embedded liver tissues of both mice and rats. Using the immunohistochemical method, we detected BALDH expression diethylnitrosamine-induced hepatomas in the male Sprague-Dawley rat and in male B6C3F1 mouse hepatomas induced with either diethylnitrosamine, ethylnitrosourea or dichloroacetic acid. BALDH was also detected in three hepatoma cell culture lines which express different levels of BALDH. These results were compared to results with normal liver and hepatoma sections from the same animals and the three cell culture lines using a standard histochemical method to detect BALDH. In nearly all these tissue sections and cell cultures, expression of BALDH was detected in identical sites with either method. The diethylnitrosamine and dichloroacetic acid induction of the BALDH isozyme, as reported here, has not been reported previously and further substantiates the use of BALDH as a histochemical marker for mouse hepatocarcinogenesis. Given the few reliable histochemical markers for mouse hepatocarcinogenesis, the immunohistochemical method will be useful for further validation of BALDH as a histochemical marker for this species. Thus, BALDH expression could be detected in any number of carcinogen-induced lesions such as altered foci, nodule or hepatomas, from archived, formalin-fixed tissues of past mouse carcinogenesis studies which were based on a variety of mouse strains, carcinogens and induction protocols.
本通讯描述了醛脱氢酶（BALDH）肿瘤相关同工酶免疫组织化学检测的方法及结果。该方法相较于标准组织化学检测方法有显著改进，后者需要冷冻或轻度固定的组织，因为在小鼠和大鼠福尔马林固定石蜡包埋肝组织的细胞中检测到了BALDH表达。使用免疫组织化学方法，我们在雄性Sprague-Dawley大鼠二乙基亚硝胺诱导的肝癌以及用二乙基亚硝胺、乙基亚硝基脲或二氯乙酸诱导的雄性B6C3F1小鼠肝癌中检测到了BALDH表达。在表达不同水平BALDH的三种肝癌细胞系中也检测到了BALDH。将这些结果与使用标准组织化学方法检测BALDH的同一动物的正常肝脏和肝癌切片以及三种细胞系的结果进行了比较。在几乎所有这些组织切片和细胞培养物中，两种方法在相同部位均检测到了BALDH表达。本文报道的二乙基亚硝胺和二氯乙酸对BALDH同工酶的诱导作用此前尚未见报道，这进一步证实了BALDH作为小鼠肝癌发生组织化学标志物的用途。鉴于小鼠肝癌发生的可靠组织化学标志物较少，免疫组织化学方法将有助于进一步验证BALDH作为该物种组织化学标志物的有效性。因此，从基于多种小鼠品系、致癌物和诱导方案的既往小鼠致癌研究中存档的福尔马林固定组织中，可在任何数量的致癌物诱导病变（如改变灶、结节或肝癌）中检测到BALDH表达。
