Richmond R E, Pereira M A
Cancer Lett. 1986 May;31(2):205-11. doi: 10.1016/0304-3835(86)90012-1.
The induction of an aldehyde dehydrogenase isozyme (B-ALDH), which uses aromatic aldehydes such as benzaldehyde as substrate and NADP as a cofactor was examined in normal liver and tumors of B6C3F1 male mice. The tumors were induced by either 15 or 45 ppm diethylnitrosamine (DENA) in the drinking water for 4 weeks. In some cases, the DENA treatment was followed with 500 ppm phenobarbital (PB) in the drinking water. DENA increased the percent of the mice that showed B-ALDH activity in their livers 14 weeks after termination of DENA treatment. The B-ALDH activity was confined mainly in the centrilobular region. Fifty weeks after the termination of the DENA treatment the percent of animals with B-ALDH in their livers was not different from control mice. However, a larger number of the tumors exhibited B-ALDH activity. These studies show that B-ALDH is a good histochemical marker for mouse liver tumors and may be useful as a marker for the effect of chemical carcinogens in this species.
在B6C3F1雄性小鼠的正常肝脏和肿瘤中，研究了一种醛脱氢酶同工酶（B-ALDH）的诱导情况，该同工酶以苯甲醛等芳香醛为底物，以NADP为辅因子。通过在饮用水中添加15或45 ppm的二乙基亚硝胺（DENA）诱导肿瘤4周。在某些情况下，DENA处理后在饮用水中添加500 ppm的苯巴比妥（PB）。DENA处理终止14周后，肝脏中出现B-ALDH活性的小鼠百分比增加。B-ALDH活性主要局限于小叶中央区域。DENA处理终止50周后，肝脏中有B-ALDH的动物百分比与对照小鼠无差异。然而，大量肿瘤表现出B-ALDH活性。这些研究表明，B-ALDH是小鼠肝脏肿瘤的良好组织化学标志物，可能作为该物种化学致癌物作用的标志物。
