Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
Plant Signal Behav. 2012 Sep 1;7(9):1138-43. doi: 10.4161/psb.21343. Epub 2012 Aug 17.
The DEAD-box RNA helicase family comprise enzymes that participate in every aspect of RNA metabolism, associated with a diverse range of cellular functions including response to abiotic stress. In the present study, we report on the identification of a new DEAD-box helicase ATP-binding protein (OsABP) from rice which is upregulated in response e to multiple abiotic stress treatments including NaCl, dehydration, ABA, blue and red light. It possesses an ORF of 2772 nt, encoding a protein of 923 aa, which contains the DEAD and helicase C-terminal domains, along with the nine conserved motifs specific to DEAD-box helicases. The in silico putative interaction with other proteins showed that OsABP interacts with proteins involved in RNA metabolism, signal transduction or stress response. These results imply that OsABP might perform important functions in the cellular response to specific abiotic stress.
DEAD-box RNA 解旋酶家族包含参与 RNA 代谢各个方面的酶，与包括对非生物胁迫的响应在内的多种细胞功能相关。在本研究中，我们报道了从水稻中鉴定出一种新的 DEAD-box 解旋酶 ATP 结合蛋白（OsABP），它在多种非生物胁迫处理包括 NaCl、脱水、ABA、蓝光和红光下被上调。它具有一个 2772 个核苷酸的 ORF，编码一个 923 个氨基酸的蛋白质，其中包含 DEAD 和解旋酶 C 末端结构域，以及九个特异性 DEAD-box 解旋酶的保守基序。与其他蛋白质的计算机预测相互作用表明，OsABP 与参与 RNA 代谢、信号转导或应激反应的蛋白质相互作用。这些结果表明，OsABP 可能在细胞对特定非生物胁迫的响应中发挥重要功能。
