Doherty S J, Fox A, Fink D W
Merck Sharp & Dohme Research Laboratories, Rahway, NJ 07065.
J Assoc Off Anal Chem. 1990 Nov-Dec;73(6):931-4.
An analytical method has been developed that is applicable to the determination of Ivermectin in medicated feeds at the 2 ppm concentration level. It is based upon liquid chromatographic analysis with a reverse-phase column and ultraviolet detection. After the drug is extracted from the feed into methanol, an analytical sample is prepared by the consecutive use of column chromatography on alumina and solid-phase extraction on Sep-Pak C18 and silica cartridges. This procedure has been applied to the concentration range 0.50-3.0 ppm of Ivermectin in feed with an accuracy of +/- 2% mean relative error and a precision of +/- 2% relative standard deviation at the 2 ppm concentration level.
已开发出一种分析方法，适用于测定药物饲料中浓度为2 ppm的伊维菌素。该方法基于使用反相柱和紫外检测的液相色谱分析。药物从饲料中萃取到甲醇后，通过依次在氧化铝上进行柱色谱以及在Sep-Pak C18和硅胶柱上进行固相萃取来制备分析样品。此程序已应用于饲料中伊维菌素浓度范围为0.50 - 3.0 ppm的情况，在2 ppm浓度水平下，平均相对误差精度为±2%，相对标准偏差精密度为±2%。
