Shah G, Bradley D, Shek E
J Assoc Off Anal Chem. 1984 Jul-Aug;67(4):707-14.
A relatively simple analytical method is presented for determination of oxfendazole (2-(methoxycarbonylamino)-5-phenylsulfinyl-benzimidazole) at levels as low as 0.012% in swine feeds, using cation exchange liquid chromatography (LC). The sample was extracted with a solvent mixture of methanol-glacial acetic acid (90 + 10) at 45 degrees C, using a gyrorotory shaker. Plant pigments and other feed excipients were removed using zinc acetate treatment and pH-controlled extraction. Oxfendazole was further separated from the remaining interferences and quantitatively determined by LC on a Partisil SCX column with acetonitrile-0.01M phosphate buffer as mobile phase. The method is stability-specific, linear, precise, and accurate at 80-120% labeled strength (relative standard deviation 0.9-1.7 with mean recovery of 98-99%). Supporting data at a level of 0.0135% oxfendazole in swine feed indicated that this method is capable of complete recovery of oxfendazole from medicated swine feeds.
本文介绍了一种相对简单的分析方法，用于使用阳离子交换液相色谱法（LC）测定猪饲料中低至0.012%的奥芬达唑（2-（甲氧基羰基氨基）-5-苯基亚磺酰基苯并咪唑）。样品在45℃下用甲醇-冰醋酸（90 + 10）的混合溶剂萃取，使用旋转振荡器。通过醋酸锌处理和pH控制萃取去除植物色素和其他饲料辅料。奥芬达唑进一步与其余干扰物分离，并在Partisil SCX柱上以乙腈-0.01M磷酸盐缓冲液为流动相通过LC进行定量测定。该方法具有稳定性特异性、线性、精密度高，在80-120%标示量时准确（相对标准偏差为0.9-1.7，平均回收率为98-99%）。猪饲料中奥芬达唑含量为0.0135%时的支持数据表明，该方法能够从加药猪饲料中完全回收奥芬达唑。
