Department of Chemistry, Indian Institute of Technology Madras, Chennai 600036, India.
Analyst. 2012 Oct 7;137(19):4559-63. doi: 10.1039/c2an35655d.
Tulsi or Holy Basil (Ocimum sanctum Linn) is a medicinally important plant. Ursolic acid (UA) and oleanolic acid (OA) are among its major constituents which account for many medicinal activities of the plant. In the present work, we deployed a new ambient ionization method, leaf spray ionization, for rapid detection of UA, OA and their oxidation products from tulsi leaves. Tandem electrospray ionization mass spectrometry (ESI-MS) has been performed on tulsi leaf extracts in methanol to establish the identity of the compounds. We probed changes occurring in the relative amounts of the parent compounds (UA and OA) with their oxidized products and the latter show an increasing trend upon ageing. The findings are verified by ESI-MS analysis of tulsi leaf extracts, which shows the same trend proving the reliability of the leaf spray method.
檀香或神圣罗勒（Ocimum sanctum Linn）是一种具有重要药用价值的植物。熊果酸（UA）和齐墩果酸（OA）是其主要成分之一，这些成分赋予了植物许多药用功效。在本工作中，我们采用了一种新的环境离子化方法，即叶片喷雾离子化，用于快速检测罗勒叶片中的 UA、OA 及其氧化产物。对甲醇中的罗勒叶提取物进行串联电喷雾电离质谱（ESI-MS）分析，以确定化合物的身份。我们研究了母体化合物（UA 和 OA）与其氧化产物的相对含量变化，结果表明后者随着老化而呈上升趋势。ESI-MS 分析罗勒叶提取物的结果验证了这一发现，证明了叶片喷雾方法的可靠性。
