Faculty of Science, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamilnadu, India.
Toxicol Mech Methods. 2012 Sep;22(7):568-76. doi: 10.3109/15376516.2012.707255.
The aim of the study was to investigate the antiinflammatory effects of naringenin in rats induced liver damage by exposure to ethanol. Rats were divided into four groups, groups 1 and 2 received isocaloric glucose; groups 3 and 4 received 20% ethanol equivalent to 6 g/kg body weight everyday for the total experimental period of 60 days. In addition, groups 2 and 4 were supplemented with naringenin (50 mg/kg p.o.) everyday for the last 30 days of the experiment. The results showed significantly elevated levels/activities/expression of serum aspartate and alanine transaminases, iron, ferritin, transforming growth factor-alpha (TNF-α), interleukin-6 (IL-6), nuclear factor-kappa B (NF-κB), cyclooxygenase-2 (COX-2), macrophage inflammatory protein 2 (MIP-2) and CD14 in ethanol fed rats as compared to those of the control. Ethanol-fed rats exhibited increased staining for the presence of inducible nitric oxide (iNOS) protein adducts in the liver. Supplementation with naringenin for the last 30 days to ethanol-fed rats, significantly decreased the levels/activities/expression of serum aspartate and alanine transaminases, iron, ferritin, TNF-α, IL-6, NF-κB, COX-2, MIP-2, CD14 and iNOS protein adducts in the liver as compared to the untreated ethanol fed rats. The inhibition of TNF-α, IL-6, NF-κB, COX-2, MIP-2, iNOS and CD14 by naringenin may contribute to its antiinflammatory activity in ethanol fed rats.
本研究旨在探讨柚皮素对乙醇诱导的大鼠肝损伤的抗炎作用。大鼠分为四组，1 组和 2 组给予等热量葡萄糖；3 组和 4 组给予 20%乙醇，相当于 6 g/kg 体重，实验总周期为 60 天。此外，2 组和 4 组在实验的最后 30 天每天补充柚皮素（50mg/kg，po）。结果显示，与对照组相比，乙醇喂养的大鼠血清天冬氨酸转氨酶和丙氨酸转氨酶、铁、铁蛋白、转化生长因子-α（TNF-α）、白细胞介素-6（IL-6）、核因子-κB（NF-κB）、环氧化酶-2（COX-2）、巨噬细胞炎症蛋白 2（MIP-2）和 CD14 的水平/活性/表达显著升高。乙醇喂养的大鼠肝脏中诱导型一氧化氮合酶（iNOS）蛋白加合物的存在染色增加。最后 30 天补充柚皮素可显著降低乙醇喂养大鼠血清天冬氨酸转氨酶和丙氨酸转氨酶、铁、铁蛋白、TNF-α、IL-6、NF-κB、COX-2、MIP-2、CD14 和 iNOS 蛋白加合物的水平/活性/表达与未治疗的乙醇喂养大鼠相比。柚皮素对 TNF-α、IL-6、NF-κB、COX-2、MIP-2、iNOS 和 CD14 的抑制作用可能与其在乙醇喂养大鼠中的抗炎活性有关。
