Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria.
Department of Biological Sciences, Faculty of Agriculture, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria.
Int J Mol Sci. 2023 Nov 13;24(22):16268. doi: 10.3390/ijms242216268.
Vitamin K (menadione), classified as a pro-vitamin, is a synthetic form of the fat-soluble family of vitamin K compounds. The combination of the vitamin with other molecules sharing structural and/or functional similarities, such as naturally occurring polyphenols, vitamins, or biopolymers, could potentiate mutual improvement of their antioxidant activity. The aim of the present study was to evaluate the role and contribution of vitamin K to the in vitro radical scavenging capacity of double and triple combinations with the phytochemicals naringenin and lignin, as well as assess possible intermolecular interactions between the bioactive compounds. Comparative analyses of the DPPH and ABTS radical scavenging activity of the pure substances vitamin K, naringenin, and lignin; the two-component systems lignin/vitamin K and vitamin K/naringenin; and the triple combination vitamin K/flavonoid/lignin were carried out. The experimental results demonstrated increased DPPH and ABTS activities of the vitamin in combination with lignin compared to those of the two pure substances, i.e., a synergistic effect was observed. The registered significant increases in the radical scavenging activity of the triple combination determined via both methods are indicative of a remarkable potentiation effect, i.e., higher antioxidant potential exceeding the additive activity of the three pure substances.
维生素 K（甲萘醌），被归类为维生素原，是脂溶性维生素 K 化合物家族的一种合成形式。维生素与其他具有结构和/或功能相似的分子（如天然存在的多酚、维生素或生物聚合物）结合，可以增强彼此的抗氧化活性。本研究的目的是评估维生素 K 在与植物化学物质柚皮素和木质素的双重和三重组合中的体外清除自由基能力中的作用和贡献，并评估生物活性化合物之间可能存在的分子间相互作用。对纯物质维生素 K、柚皮素和木质素；两种成分系统木质素/维生素 K 和维生素 K/柚皮素；以及维生素 K/类黄酮/木质素的三重组合进行了 DPPH 和 ABTS 自由基清除活性的比较分析。实验结果表明，与两种纯物质相比，维生素与木质素结合后 DPPH 和 ABTS 活性增加，即观察到协同作用。通过两种方法确定的三重组合的自由基清除活性的显著增加表明存在显著的增效作用，即具有更高的抗氧化潜力，超过三种纯物质的相加活性。
